Isolation and screening of amylase activity of primary moulds in Ragi Tapai of Indonesia

R. A. Anasa, W. Nurlaila, W. Dharmastuti, I. Santoso, A. E. Maryanto, S. Sitaresmi, Y. Yasman

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Primary moulds involved in tapai fermentation have been isolated on Potato Dextrose Agar (PDA) supplemented with tetracycline. Fifteen different ragi tapai samples used in the experiment were collected from West Java, Central Java and outside Java. A total of 56 mould isolates have been isolated consisting of 13 isolates from ragi tapai of West Java, 21 isolates from ragi tapai of Central Java, and 22 isolates from ragi tapai of outside Java. The isolates were cultured on PDA medium and screened. Amylase activity was determined by iodine method and measured for the diameter of clear zone. Based on screening of amylase activity, isolates ZC1 (69.95 mm) from ragi tapai of West Java, ZSL3 (58.1 mm) from ragi tapai of Central Java, and ZMDN1 (85.00 mm) from ragi tapai of outside Java were the most potential showing the widest diameter of clear zone. Macroscopic and microscopic observation of the isolates showed isolates ZC1 and ZSL3 belong to genus Rhizopus while isolate ZMDN1 belongs to genus Amylomyces.

Original languageEnglish
Title of host publicationProceedings of the 4th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2018
EditorsTerry Mart, Djoko Triyono, Ivandini T. Anggraningrum
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735419155
DOIs
Publication statusPublished - 4 Nov 2019
Event4th International Symposium on Current Progress in Mathematics and Sciences 2018, ISCPMS 2018 - Depok, Indonesia
Duration: 30 Oct 201831 Oct 2018

Publication series

NameAIP Conference Proceedings
Volume2168
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference4th International Symposium on Current Progress in Mathematics and Sciences 2018, ISCPMS 2018
CountryIndonesia
CityDepok
Period30/10/1831/10/18

Keywords

  • Amylase activity
  • Isolation
  • Primary moulds
  • Ragi tapai
  • Screening

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    Anasa, R. A., Nurlaila, W., Dharmastuti, W., Santoso, I., Maryanto, A. E., Sitaresmi, S., & Yasman, Y. (2019). Isolation and screening of amylase activity of primary moulds in Ragi Tapai of Indonesia. In T. Mart, D. Triyono, & I. T. Anggraningrum (Eds.), Proceedings of the 4th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2018 [020080] (AIP Conference Proceedings; Vol. 2168). American Institute of Physics Inc.. https://doi.org/10.1063/1.5132507