@inproceedings{60624d4be3b04f3b86c4111eed9674d3,
title = "Isolation and screening of amylase activity of primary moulds in Ragi Tapai of Indonesia",
abstract = "Primary moulds involved in tapai fermentation have been isolated on Potato Dextrose Agar (PDA) supplemented with tetracycline. Fifteen different ragi tapai samples used in the experiment were collected from West Java, Central Java and outside Java. A total of 56 mould isolates have been isolated consisting of 13 isolates from ragi tapai of West Java, 21 isolates from ragi tapai of Central Java, and 22 isolates from ragi tapai of outside Java. The isolates were cultured on PDA medium and screened. Amylase activity was determined by iodine method and measured for the diameter of clear zone. Based on screening of amylase activity, isolates ZC1 (69.95 mm) from ragi tapai of West Java, ZSL3 (58.1 mm) from ragi tapai of Central Java, and ZMDN1 (85.00 mm) from ragi tapai of outside Java were the most potential showing the widest diameter of clear zone. Macroscopic and microscopic observation of the isolates showed isolates ZC1 and ZSL3 belong to genus Rhizopus while isolate ZMDN1 belongs to genus Amylomyces.",
keywords = "Amylase activity, Isolation, Primary moulds, Ragi tapai, Screening",
author = "Anasa, {R. A.} and W. Nurlaila and W. Dharmastuti and I. Santoso and Maryanto, {A. E.} and S. Sitaresmi and Y. Yasman",
note = "Funding Information: The research was supported by the Ministries of Research, Technology, and Higher Education Republic of Indonesia of Grant research Penelitian Terapan Unggulan Perguruan Tinggi 2018 (PTUPT 2018) with grant number: 476/UN2.R3.1/HKP05.00/2018. The authors are grateful for collaboration with the Center of Excellence for Indigenous Biological Resources-Genome Studies (CoE IBR-GS) FMIPA UI. Publisher Copyright: {\textcopyright} 2019 Author(s).; 4th International Symposium on Current Progress in Mathematics and Sciences 2018, ISCPMS 2018 ; Conference date: 30-10-2018 Through 31-10-2018",
year = "2019",
month = nov,
day = "4",
doi = "10.1063/1.5132507",
language = "English",
series = "AIP Conference Proceedings",
publisher = "American Institute of Physics Inc.",
editor = "Terry Mart and Djoko Triyono and Anggraningrum, {Ivandini T.}",
booktitle = "Proceedings of the 4th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2018",
}