Isolation and screening antimicrobial activity of actinomycetes from sediment's coastal Pramuka Island, Kepulauan Seribu, Jakarta, Indonesia

R. Alfisyahri, Iman Santoso, Yasman

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

3 Citations (Scopus)

Abstract

Twenty-two isolates of actinomycetes have been isolated from sediment in coastal Pramuka Island, Kepulauan Seribu, Jakarta, Indonesia. Plate method was used for isolating actinomycetes on Zhang' Starch Soil Extract (ZSSE). All of the isolates were grown in Cross Streak Medium (CSM) agar plate. Strains were screened for antimicrobial activity by using plug method and their activities were determined by antimicrobial Activity Index (AI). The result showed that 13 isolates inhibited Staphylococcus aureus NBRC 100910 (0.461 - 2.338), 19 isolates inhibited Kocuria rhizophila NBRC 12708 (0.705 - 8.200), five isolates inhibited Candida albicans UICC Y29 (0.885 - 0.357), and four isolates inhibited Saccharomyces cerivisiae UICC Y17 (0.357 - 1.348). No inhibition activity was observed in the Escherichia coli NBRC 3301. Based on the results of antimicrobial test, SD17 is the most potential strain since it is able to inhibit all Gram positive and yeast tested.

Original languageEnglish
Title of host publicationProceedings of the 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
EditorsRatna Yuniati, Terry Mart, Ivandini T. Anggraningrum, Djoko Triyono, Kiki A. Sugeng
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735417410
DOIs
Publication statusPublished - 22 Oct 2018
Event3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017 - Bali, Indonesia
Duration: 26 Jul 201727 Jul 2017

Publication series

NameAIP Conference Proceedings
Volume2023
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
Country/TerritoryIndonesia
CityBali
Period26/07/1727/07/17

Keywords

  • Actinomycetes
  • Antimicrobial screening
  • Coastal sediment
  • Isolation
  • Pramuka Island

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