Isolation and screening antibacterial activity of actinomycetes from mangrove ecosystem, Pramuka Island, Kepulauan Seribu, Jakarta, Indonesia

Q. G. Fadhilah, Iman Santoso, Yasman

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

5 Citations (Scopus)

Abstract

Thirty-eight isolates of actinomycetes have been isolated from samples which were collected from mangrove ecosystem in Pramuka Island, Kepulauan Seribu, Jakarta, Indonesia. Isolation was done by using plate method on Zhang' Starch Soil Extract (ZSSE) agar. The pure cultures were grown in Cross Streak Medium (CSM) agar plate and subjected for antibacterial activity. Antibacterial activity was screened using plug method against Gram negative Escherichia coli NBRC 3301, Gram positive Staphylococcus aureus NBRC 100910 and Kocuria rhizophila NBRC 12708 then was determined by antibacterial Activity Index (AI). The result showed that three isolates inhibited Escherichia coli NBRC 3301 (0.408 - 0.708), 18 isolates inhibited Staphylococcus aureus NBRC 100910 (0.977 - 3.482), and 17 isolates inhibited Kocuria rhizophila NBRC 12708 (1.182 - 6.814). Among the strains tested, SRM 2 and SRM 13 were the most potential strains because both give positive result to all tested bacteria.

Original languageEnglish
Title of host publicationProceedings of the 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
EditorsRatna Yuniati, Terry Mart, Ivandini T. Anggraningrum, Djoko Triyono, Kiki A. Sugeng
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735417410
DOIs
Publication statusPublished - 22 Oct 2018
Event3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017 - Bali, Indonesia
Duration: 26 Jul 201727 Jul 2017

Publication series

NameAIP Conference Proceedings
Volume2023
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
Country/TerritoryIndonesia
CityBali
Period26/07/1727/07/17

Keywords

  • Actinomycetes
  • Antibacterial screening
  • Isolation
  • Mangrove ecosystem
  • Pramuka island

Fingerprint

Dive into the research topics of 'Isolation and screening antibacterial activity of actinomycetes from mangrove ecosystem, Pramuka Island, Kepulauan Seribu, Jakarta, Indonesia'. Together they form a unique fingerprint.

Cite this