Isolation and purification of bromelain from pineapple core (Ananas comosus [L]. Merr) by ammonium sulfate and ethanol precipitation

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

This study was conducted on bromelain from pineapple core (Ananas comosus [L] Merr). The crude enzyme gained from pineapple core extract was then fractionated by (NH4)2SO4 and ethanol in different concentration levels. Fractionation using 20-50% (NH4)2SO4 resulted in the highest specific activity of 89.32 U/mg with a purity level of 1.72 times. Further purification increased its specific activity to 120.83 U/mg with a purity level of 2.32 times. Fractionation using 20-50% ethanol obtained the highest specific activity of 184.79 U/mg with a purity level of 27.15 times. Bromelain fractions with the highest specific activity were tested in vitro for their activity as antiplatelet agents using PRP (Platelet Rich Plasma). The platelet aggregation percentage of ammonium sulfate fraction was shown as 17.12% with the platelet aggregation inhibition percentage of 81.56%. The 20-50% ethanol fraction showed the platelet aggregation percentage was 15.93% with the percentage of platelet aggregation inhibition of 82.83%. The fraction of bromelain with higher specific activity showed a higher aggregate antiplatelet activity.

Original languageEnglish
Title of host publicationProceedings of the 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
EditorsRatna Yuniati, Terry Mart, Ivandini T. Anggraningrum, Djoko Triyono, Kiki A. Sugeng
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735417410
DOIs
Publication statusPublished - 22 Oct 2018
Event3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017 - Bali, Indonesia
Duration: 26 Jul 201727 Jul 2017

Publication series

NameAIP Conference Proceedings
Volume2023
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
CountryIndonesia
CityBali
Period26/07/1727/07/17

Fingerprint Dive into the research topics of 'Isolation and purification of bromelain from pineapple core (Ananas comosus [L]. Merr) by ammonium sulfate and ethanol precipitation'. Together they form a unique fingerprint.

  • Cite this

    Sari, A. A., Setiasih, S., Pws, S. H., & Saefudin, E. (2018). Isolation and purification of bromelain from pineapple core (Ananas comosus [L]. Merr) by ammonium sulfate and ethanol precipitation. In R. Yuniati, T. Mart, I. T. Anggraningrum, D. Triyono, & K. A. Sugeng (Eds.), Proceedings of the 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017 [020076] (AIP Conference Proceedings; Vol. 2023). American Institute of Physics Inc.. https://doi.org/10.1063/1.5064073