Isolation and purification of bromelain from pineapple core (Ananas comosus [L]. Merr) by ammonium sulfate and ethanol precipitation

A. A. Sari; S. Setiasih; S. Hudiyono; E. Saepudin

doi:10.1063/1.5064073

Isolation and purification of bromelain from pineapple core (Ananas comosus [L]. Merr) by ammonium sulfate and ethanol precipitation

A. A. Sari, S. Setiasih, S. Hudiyono, E. Saepudin

Department of Chemistry

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

5 Citations (Scopus)

Abstract

This study was conducted on bromelain from pineapple core (Ananas comosus [L] Merr). The crude enzyme gained from pineapple core extract was then fractionated by (NH₄)₂SO₄ and ethanol in different concentration levels. Fractionation using 20-50% (NH₄)₂SO₄ resulted in the highest specific activity of 89.32 U/mg with a purity level of 1.72 times. Further purification increased its specific activity to 120.83 U/mg with a purity level of 2.32 times. Fractionation using 20-50% ethanol obtained the highest specific activity of 184.79 U/mg with a purity level of 27.15 times. Bromelain fractions with the highest specific activity were tested in vitro for their activity as antiplatelet agents using PRP (Platelet Rich Plasma). The platelet aggregation percentage of ammonium sulfate fraction was shown as 17.12% with the platelet aggregation inhibition percentage of 81.56%. The 20-50% ethanol fraction showed the platelet aggregation percentage was 15.93% with the percentage of platelet aggregation inhibition of 82.83%. The fraction of bromelain with higher specific activity showed a higher aggregate antiplatelet activity.

Original language	English
Title of host publication	Proceedings of the 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
Editors	Ratna Yuniati, Terry Mart, Ivandini T. Anggraningrum, Djoko Triyono, Kiki A. Sugeng
Publisher	American Institute of Physics Inc.
ISBN (Electronic)	9780735417410
DOIs	https://doi.org/10.1063/1.5064073
Publication status	Published - 22 Oct 2018
Event	3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017 - Bali, Indonesia Duration: 26 Jul 2017 → 27 Jul 2017

Publication series

Name	AIP Conference Proceedings
Volume	2023
ISSN (Print)	0094-243X
ISSN (Electronic)	1551-7616

Conference

Conference	3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
Country/Territory	Indonesia
City	Bali
Period	26/07/17 → 27/07/17

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10.1063/1.5064073

Cite this

Sari, A. A., Setiasih, S., Hudiyono, S., & Saepudin, E. (2018). Isolation and purification of bromelain from pineapple core (Ananas comosus [L]. Merr) by ammonium sulfate and ethanol precipitation. In R. Yuniati, T. Mart, I. T. Anggraningrum, D. Triyono, & K. A. Sugeng (Eds.), Proceedings of the 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017 Article 020076 (AIP Conference Proceedings; Vol. 2023). American Institute of Physics Inc.. https://doi.org/10.1063/1.5064073

Sari, A. A. ; Setiasih, S. ; Hudiyono, S. et al. / Isolation and purification of bromelain from pineapple core (Ananas comosus [L]. Merr) by ammonium sulfate and ethanol precipitation. Proceedings of the 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017. editor / Ratna Yuniati ; Terry Mart ; Ivandini T. Anggraningrum ; Djoko Triyono ; Kiki A. Sugeng. American Institute of Physics Inc., 2018. (AIP Conference Proceedings).

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title = "Isolation and purification of bromelain from pineapple core (Ananas comosus [L]. Merr) by ammonium sulfate and ethanol precipitation",

abstract = "This study was conducted on bromelain from pineapple core (Ananas comosus [L] Merr). The crude enzyme gained from pineapple core extract was then fractionated by (NH4)2SO4 and ethanol in different concentration levels. Fractionation using 20-50% (NH4)2SO4 resulted in the highest specific activity of 89.32 U/mg with a purity level of 1.72 times. Further purification increased its specific activity to 120.83 U/mg with a purity level of 2.32 times. Fractionation using 20-50% ethanol obtained the highest specific activity of 184.79 U/mg with a purity level of 27.15 times. Bromelain fractions with the highest specific activity were tested in vitro for their activity as antiplatelet agents using PRP (Platelet Rich Plasma). The platelet aggregation percentage of ammonium sulfate fraction was shown as 17.12% with the platelet aggregation inhibition percentage of 81.56%. The 20-50% ethanol fraction showed the platelet aggregation percentage was 15.93% with the percentage of platelet aggregation inhibition of 82.83%. The fraction of bromelain with higher specific activity showed a higher aggregate antiplatelet activity.",

author = "Sari, {A. A.} and S. Setiasih and S. Hudiyono and E. Saepudin",

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Sari, AA, Setiasih, S, Hudiyono, S & Saepudin, E 2018, Isolation and purification of bromelain from pineapple core (Ananas comosus [L]. Merr) by ammonium sulfate and ethanol precipitation. in R Yuniati, T Mart, IT Anggraningrum, D Triyono & KA Sugeng (eds), Proceedings of the 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017., 020076, AIP Conference Proceedings, vol. 2023, American Institute of Physics Inc., 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017, Bali, Indonesia, 26/07/17. https://doi.org/10.1063/1.5064073

Isolation and purification of bromelain from pineapple core (Ananas comosus [L]. Merr) by ammonium sulfate and ethanol precipitation. / Sari, A. A.; Setiasih, S.; Hudiyono, S. et al.
Proceedings of the 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017. ed. / Ratna Yuniati; Terry Mart; Ivandini T. Anggraningrum; Djoko Triyono; Kiki A. Sugeng. American Institute of Physics Inc., 2018. 020076 (AIP Conference Proceedings; Vol. 2023).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Isolation and purification of bromelain from pineapple core (Ananas comosus [L]. Merr) by ammonium sulfate and ethanol precipitation

AU - Sari, A. A.

AU - Setiasih, S.

AU - Hudiyono, S.

AU - Saepudin, E.

PY - 2018/10/22

Y1 - 2018/10/22

N2 - This study was conducted on bromelain from pineapple core (Ananas comosus [L] Merr). The crude enzyme gained from pineapple core extract was then fractionated by (NH4)2SO4 and ethanol in different concentration levels. Fractionation using 20-50% (NH4)2SO4 resulted in the highest specific activity of 89.32 U/mg with a purity level of 1.72 times. Further purification increased its specific activity to 120.83 U/mg with a purity level of 2.32 times. Fractionation using 20-50% ethanol obtained the highest specific activity of 184.79 U/mg with a purity level of 27.15 times. Bromelain fractions with the highest specific activity were tested in vitro for their activity as antiplatelet agents using PRP (Platelet Rich Plasma). The platelet aggregation percentage of ammonium sulfate fraction was shown as 17.12% with the platelet aggregation inhibition percentage of 81.56%. The 20-50% ethanol fraction showed the platelet aggregation percentage was 15.93% with the percentage of platelet aggregation inhibition of 82.83%. The fraction of bromelain with higher specific activity showed a higher aggregate antiplatelet activity.

AB - This study was conducted on bromelain from pineapple core (Ananas comosus [L] Merr). The crude enzyme gained from pineapple core extract was then fractionated by (NH4)2SO4 and ethanol in different concentration levels. Fractionation using 20-50% (NH4)2SO4 resulted in the highest specific activity of 89.32 U/mg with a purity level of 1.72 times. Further purification increased its specific activity to 120.83 U/mg with a purity level of 2.32 times. Fractionation using 20-50% ethanol obtained the highest specific activity of 184.79 U/mg with a purity level of 27.15 times. Bromelain fractions with the highest specific activity were tested in vitro for their activity as antiplatelet agents using PRP (Platelet Rich Plasma). The platelet aggregation percentage of ammonium sulfate fraction was shown as 17.12% with the platelet aggregation inhibition percentage of 81.56%. The 20-50% ethanol fraction showed the platelet aggregation percentage was 15.93% with the percentage of platelet aggregation inhibition of 82.83%. The fraction of bromelain with higher specific activity showed a higher aggregate antiplatelet activity.

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M3 - Conference contribution

AN - SCOPUS:85056127165

T3 - AIP Conference Proceedings

BT - Proceedings of the 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017

A2 - Yuniati, Ratna

A2 - Mart, Terry

A2 - Anggraningrum, Ivandini T.

A2 - Triyono, Djoko

A2 - Sugeng, Kiki A.

PB - American Institute of Physics Inc.

T2 - 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017

Y2 - 26 July 2017 through 27 July 2017

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Sari AA, Setiasih S, Hudiyono S, Saepudin E. Isolation and purification of bromelain from pineapple core (Ananas comosus [L]. Merr) by ammonium sulfate and ethanol precipitation. In Yuniati R, Mart T, Anggraningrum IT, Triyono D, Sugeng KA, editors, Proceedings of the 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017. American Institute of Physics Inc. 2018. 020076. (AIP Conference Proceedings). doi: 10.1063/1.5064073

Isolation and purification of bromelain from pineapple core (Ananas comosus [L]. Merr) by ammonium sulfate and ethanol precipitation

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