TY - GEN
T1 - Isolation and optimization of polyhydroxybutyrate from Chlorella vulgaris using NaClO and ultrasonication aided chemical pretreatment methods
AU - Setyorini, Ajeng Inas
AU - Dianursanti,
N1 - Funding Information:
The authors would like to thank the Laboratory of Bioprocess and Laboratory of Basic Chemical Process in Chemical Engineering Department, Faculty of Engineering for their research facilities, and towards Directorate of Research and Community Engagement Universitas Indonesia (DRPM UI) through Publikasi Terindeks Internasional (PUTI) Prosiding 2020 NKB-1145/UN2.RST/HKP.05.00/2020 for providing financial support on this research.
Publisher Copyright:
© 2021 Author(s).
PY - 2021/3/23
Y1 - 2021/3/23
N2 - The increasing use of food packaging makes plastic waste the biggest environmental issue. Since the use of conventional plastic comes from fossil polymers, so it is difficult to be decomposed by bacteria. The right solution is to replace it with bioplastics. This study uses Chlorella vulgaris as a producer of Poly-ß-hydroxybutyrate (PHB). PHB has characteristics such as biodegradability, thermoplasticity, hydrophobicity, biocompatibility with cells and tissues, and physical properties similar to polypropylene, which can potentially apply in food, pharmaceutical, and medical packaging. PHB is obtained by isolating biomass from Chlorella vulgaris. This research studied PHB isolation using chemical and mechanical cell disruption methods, namely NaClO and sonication, with variations in solvent concentration. The simplest and most economical method of isolation of C. vulgaris is the general stages of cell disruption, PHB precipitation, and PHB purification. The quantifying PHB method is by calculating the yield of PHB, then identifying PHB by FTIR, and testing the quality of the best PHB compounds by GCMS. The parameters used in this study are yield, PHB physical results, and PHB test results. In the condition of 0.6% w/v sample concentration and 1.37% w/v solvent concentration using ultrasonication, the best PHB isolation yield was 37.2%. GCMS sample results showed the presence of n-hexadecanoic acid (palmitic acid), 9-Octadecanoic acid (oleic acid), 2-Palmitoylglycerol, and Octadecanoic acid (stearic acid) as the main compounds that could potentially be bioplastic materials.
AB - The increasing use of food packaging makes plastic waste the biggest environmental issue. Since the use of conventional plastic comes from fossil polymers, so it is difficult to be decomposed by bacteria. The right solution is to replace it with bioplastics. This study uses Chlorella vulgaris as a producer of Poly-ß-hydroxybutyrate (PHB). PHB has characteristics such as biodegradability, thermoplasticity, hydrophobicity, biocompatibility with cells and tissues, and physical properties similar to polypropylene, which can potentially apply in food, pharmaceutical, and medical packaging. PHB is obtained by isolating biomass from Chlorella vulgaris. This research studied PHB isolation using chemical and mechanical cell disruption methods, namely NaClO and sonication, with variations in solvent concentration. The simplest and most economical method of isolation of C. vulgaris is the general stages of cell disruption, PHB precipitation, and PHB purification. The quantifying PHB method is by calculating the yield of PHB, then identifying PHB by FTIR, and testing the quality of the best PHB compounds by GCMS. The parameters used in this study are yield, PHB physical results, and PHB test results. In the condition of 0.6% w/v sample concentration and 1.37% w/v solvent concentration using ultrasonication, the best PHB isolation yield was 37.2%. GCMS sample results showed the presence of n-hexadecanoic acid (palmitic acid), 9-Octadecanoic acid (oleic acid), 2-Palmitoylglycerol, and Octadecanoic acid (stearic acid) as the main compounds that could potentially be bioplastic materials.
KW - Chlorella vulgaris
KW - NaClO
KW - polyhydroxybutyrate (PHB)
KW - solvent extraction
KW - ultrasonication
UR - http://www.scopus.com/inward/record.url?scp=85103521976&partnerID=8YFLogxK
U2 - 10.1063/5.0047562
DO - 10.1063/5.0047562
M3 - Conference contribution
AN - SCOPUS:85103521976
T3 - AIP Conference Proceedings
BT - 5th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices
A2 - Lischer, Kenny
A2 - Supriadi, Sugeng
A2 - Rahman, Siti Fauziyah
A2 - Whulanza, Yudan
PB - American Institute of Physics Inc.
T2 - 5th International Symposium of Biomedical Engineering, ISBE 2020
Y2 - 28 July 2020 through 29 July 2020
ER -