TY - GEN
T1 - Isolation and morphological characterization of fungi from deteriorated old Chinese manuscripts from Central Library Universitas Indonesia
AU - Rachmania, M. K.
AU - Oetari, Ariyanti
AU - Fitri, R.
AU - Susetyo-Salim, T.
AU - Sjamsuridzal, Wellyzar
N1 - Funding Information:
The authors are very grateful to Universitas Indonesia for supporting our research through by Hibah Pascasarjana UI 2015 with contract number 1714/UN2.R12/HKP.05.00/2015 and PITTA Grant 2017 with contract number 627/UN2.R3.1/HKP.05.00/2017. The authors thank Mrs. Mariyah, S.Sos, M.Hum. and Ms. Aswinna, S. Hum. from Central Library UI for their kindly assistance.
Publisher Copyright:
© 2018 Author(s).
PY - 2018/10/22
Y1 - 2018/10/22
N2 - Fungi, which cause deterioration of old Chinese manuscripts in Indonesia, are rarely known. The collected old Chinese manuscripts of Central Library Universitas Indonesia (Perpustakaan Pusat UI) showed a possible deterioration of fungi. The aims of this study were to isolate and morphologically characterize fungi from two deteriorated old Chinese manuscripts from Ruang Naskah Central Library Universitas Indonesia. The old Chinese manuscripts were observed for signs of biodeterioration by using stereomicroscope and two manuscripts were selected. Samples from the manuscripts were collected by using swab method with sterile cotton swabs. Fungal isolates were isolated by culture-dependent method on Potato Dextrose Agar (PDA). Two deteriorated old Chinese manuscripts showed brown or black spots and spore formation on the surface of manuscripts. Six fungal isolates were selected based on morphological type differences according to the monographs. Based on morphological characteristics, five fungal isolates from the deteriorated old Chinese manuscripts belonged to four genera of the anamorphic fungi as follows: Cladosporium (1 isolate), Curvularia (2 isolates), Penicillium (1 isolate), and Sarocladium (1 isolate). One isolate could not be morphologically characterized to the genus level. This study showed that fungi from various genera could be isolated from deteriorated old Chinese manuscripts. Phylogeny-based identification using the data set of internal transcribed spacer (ITS) sequences is required to determine the species identities of the fungal strains.
AB - Fungi, which cause deterioration of old Chinese manuscripts in Indonesia, are rarely known. The collected old Chinese manuscripts of Central Library Universitas Indonesia (Perpustakaan Pusat UI) showed a possible deterioration of fungi. The aims of this study were to isolate and morphologically characterize fungi from two deteriorated old Chinese manuscripts from Ruang Naskah Central Library Universitas Indonesia. The old Chinese manuscripts were observed for signs of biodeterioration by using stereomicroscope and two manuscripts were selected. Samples from the manuscripts were collected by using swab method with sterile cotton swabs. Fungal isolates were isolated by culture-dependent method on Potato Dextrose Agar (PDA). Two deteriorated old Chinese manuscripts showed brown or black spots and spore formation on the surface of manuscripts. Six fungal isolates were selected based on morphological type differences according to the monographs. Based on morphological characteristics, five fungal isolates from the deteriorated old Chinese manuscripts belonged to four genera of the anamorphic fungi as follows: Cladosporium (1 isolate), Curvularia (2 isolates), Penicillium (1 isolate), and Sarocladium (1 isolate). One isolate could not be morphologically characterized to the genus level. This study showed that fungi from various genera could be isolated from deteriorated old Chinese manuscripts. Phylogeny-based identification using the data set of internal transcribed spacer (ITS) sequences is required to determine the species identities of the fungal strains.
UR - http://www.scopus.com/inward/record.url?scp=85056116439&partnerID=8YFLogxK
U2 - 10.1063/1.5064141
DO - 10.1063/1.5064141
M3 - Conference contribution
AN - SCOPUS:85056116439
T3 - AIP Conference Proceedings
BT - Proceedings of the 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
A2 - Yuniati, Ratna
A2 - Mart, Terry
A2 - Anggraningrum, Ivandini T.
A2 - Triyono, Djoko
A2 - Sugeng, Kiki A.
PB - American Institute of Physics Inc.
T2 - 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
Y2 - 26 July 2017 through 27 July 2017
ER -