The aims of this study were to isolate and identify the members of Ktedonobacteria from soil samples in Cisolok geysers area, West Java, Indonesia. To isolate the bacteria, sample was spread on 10-fold-diluted Reasoner's 2A medium and the plates were incubated at 30 °C until the Ktedonobacteria-like colonies appeared. Ktedonobacteria-like colonies showed the characteristics of a firm pale orange colony. The colonies were purified and colony PCR was performed using Ktedonobacteria specific and universal eubacterial primers for 16S rRNA gene. Almost full-length of 16S rRNA gene sequences were obtained in order to know the identity of the taxonomic affiliation of the isolates. Three Ktedonobacteria-like isolates, designated S188.8.131.52, S184.108.40.206 (isolated from soil under Bamboo tree) and S220.127.116.11 (isolated from soil in decayed Bamboo stems) were obtained. The detection using Ktedonobacteria specific primers showed positive results, which confirmed that all of three isolates belong to class Ktedonobacteria. The sequence homology search using EzBioCloud showed that these isolates (S18.104.22.168, S22.214.171.124, and S126.96.36.199) were belonged to the family Dictyobacteraceae and closely related to Bacterium SOSP1-79 (99.20%, 98.83%, 99.34%, respectively) and Dictyobacter aurantiacus S-27T (97.41%, 97.41%, 98.00%, respectively). The low percentage of sequence homology to type strains indicates that they could be a candidate of novel taxa of the genus Dictyobacter. Further characterizations such as morphology, physiology, and chemotaxonomy are needed in order to clarify the taxonomic position of these potential novel species of Dictyobacter.
|Journal||IOP Conference Series: Earth and Environmental Science|
|Publication status||Published - 21 Feb 2020|
|Event||6th International Symposium of Innovative Bio-Production Indonesia on Biotechnology and Bioengineering, ISIBio2019 - Tangerang, Indonesia|
Duration: 23 Oct 2019 → 24 Oct 2019