Isolation and identification of chemical compounds from garcinia fruticosa lauterb stem bark extract

Novia Delita, Berna Elya, Muhammad Hanafi

Research output: Contribution to journalArticlepeer-review


Background: Garcinia is a tropical plant that grows in Indonesia. Garcinia has many health benefits for the body. Garcinia contains many phenolic compounds and their derivatives, such as xanthon, flavonoids, benzophenone, lactone, and phenolic acids. Garcinia fruticosa Lauterb. comes from the family Clusiaceae. The results of the phytochemical examination showed that G. fruticosa bark extract contained alkaloids, flavonoids, glycosides, tannins, and saponins. Objective: This study aims to isolate and identify chemical compounds from the ethyl acetate extract of G. fruticosa Lauterb stem bark. Method: G. fruticosa Lauterb bark. dried, milled, and extracted with Step Gradient Polarity/SGP maceration using n-hexane, ethyl acetate, and methanol. Isolation was done by column chromatography and identified by thin layer chromatography and IR spectroscopy, LC-MS/MS, 1H-NMR, 13C-NMR, 2D-NMR (HSQC, HMBC). Results: Compound D7a has a molecular weight 168.0496. The IR spectrum shows the presence of a group -OH appears on 3483 cm-1aromatic presence in 1609 cm-1. The H-NMR spectrum shows the presence of aromatic signals on 6.96 (d, 8 Hz), 6.96 (d, 2 Hz) and 7.70 (dd, 8; 2 Hz). The C-NMR spectrum shows the presence of a carboxylic-COOH group appearing at 166.57 ppm, the presence of 2 x C-OH appearing at 147.18 and 151.18. In the HMBC spectrum, the -OCH3 position is located at C-3 with a correlation between the 3.79 (s) signal and the C signal at the chemical shift 147.18. Conclusions: Structural elucidation shows that compound D7a is a 4-hydroxy-3-methoxy benzoate acid (Vanylic Acid) and isolate I-1 is an impure compound namely β-Sitosterol and Stigmasterol.

Original languageEnglish
Pages (from-to)1641-1652
Number of pages12
JournalPharmacognosy Journal
Issue number6
Publication statusPublished - Nov 2020


  • 4-hydroxy-3-methoxy benzoic acid
  • Garcinia fruticosa
  • Isolation
  • Stigmasterol
  • Structural elucidation
  • β-Sitosterol


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