TY - JOUR
T1 - Isolation and complete genome analysis of neurotropic dengue virus serotype 3 from the cerebrospinal fluid of an encephalitis patient
AU - Dhenni, Rama
AU - Karyanti, Mulya Rahma
AU - Putri, Nina Dwi
AU - Yohan, Benediktus
AU - Yudhaputri, Frilasita A.
AU - Ma'roef, Chairin Nisa
AU - Fadhilah, Araniy
AU - Perkasa, Aditya
AU - Restuadi, Restuadi
AU - Trimarsanto, Hidayat
AU - Mangunatmadja, Irawan
AU - Ledermann, Jeremy P.
AU - Rosenberg, Ronald
AU - Powers, Ann M.
AU - Myint, Khin Saw Aye
AU - Sasmono, R. Tedjo
N1 - Publisher Copyright:
© 2018 Public Library of Science. All Rights Reserved.
PY - 2018/1
Y1 - 2018/1
N2 - Although neurological manifestations associated with dengue viruses (DENV) infection have been reported, there is very limited information on the genetic characteristics of neurotropic DENV. Here we describe the isolation and complete genome analysis of DENV serotype 3 (DENV-3) from cerebrospinal fluid of an encephalitis paediatric patient in Jakarta, Indonesia. Next-generation sequencing was employed to deduce the complete genome of the neurotropic DENV-3 isolate. Based on complete genome analysis, two unique and nine uncommon amino acid changes in the protein coding region were observed in the virus. A phylogenetic tree and molecular clock analysis revealed that the neurotropic virus was a member of Sumatran-Javan clade of DENV-3 genotype I and shared a common ancestor with other isolates from Jakarta around 1998. This is the first report of neurotropic DENV-3 complete genome analysis, providing detailed information on the genetic characteristics of this virus.
AB - Although neurological manifestations associated with dengue viruses (DENV) infection have been reported, there is very limited information on the genetic characteristics of neurotropic DENV. Here we describe the isolation and complete genome analysis of DENV serotype 3 (DENV-3) from cerebrospinal fluid of an encephalitis paediatric patient in Jakarta, Indonesia. Next-generation sequencing was employed to deduce the complete genome of the neurotropic DENV-3 isolate. Based on complete genome analysis, two unique and nine uncommon amino acid changes in the protein coding region were observed in the virus. A phylogenetic tree and molecular clock analysis revealed that the neurotropic virus was a member of Sumatran-Javan clade of DENV-3 genotype I and shared a common ancestor with other isolates from Jakarta around 1998. This is the first report of neurotropic DENV-3 complete genome analysis, providing detailed information on the genetic characteristics of this virus.
UR - http://www.scopus.com/inward/record.url?scp=85041727547&partnerID=8YFLogxK
U2 - 10.1371/journal.pntd.0006198
DO - 10.1371/journal.pntd.0006198
M3 - Article
C2 - 29329287
AN - SCOPUS:85041727547
SN - 1935-2727
VL - 12
JO - PLoS Neglected Tropical Diseases
JF - PLoS Neglected Tropical Diseases
IS - 1
M1 - e0006198
ER -