TY - GEN
T1 - Isolation and characterization of potential bacteria with the ability to degrade Congo Red dye
AU - Santoso, Iman
AU - Purnomo, Willy
AU - Oktavianto, Haris
AU - Sjamsuridzal, Wellyzar
AU - Handayani, Windri
AU - Sunardi,
N1 - Publisher Copyright:
© 2016 Author(s).
PY - 2016/4/19
Y1 - 2016/4/19
N2 - Bacterial isolates, which are able to degrade the azo textile dye Congo Red, have been isolated from Bantar Gebang Landfills-Bekasi and Citarum river, West Java. The initial screening using Zhou-Zimmerman medium which contains 50 ppm Congo Red yielded in 6 isolates (D2, I1, I4, L2B, L2C, and M) were able to degrade the dye. Selection for the best isolate using Bushnell-Haas medium showed that isolates L2C was able to degrade up to 98.5% of Congo Red within 3 days. The percentage of degradation was measured using Spectrophotometer at Λ 490 nm. Assessment of biodegradation profile of L2C at 500 ppm, 700 ppm and 1000 ppm of Congo Red showed that in 7 days, the isolates degraded the dye solution by 96.7%, 97.7% and 82.6%, respectively. The degrading rate of the re-isolated L2C cells at 1000 ppm of Congo Red increased up to 97.8% in 7 days when the cells were adapted into the Congo Red dye. Phytotoxicity test for germination using corn seed (Zea mays) showed that the toxicity of filtrate was slightly lower (50%) compared to the Congo Red 1000 ppm solution that is used as a control (67%). Molecular identification based on sequence 16S rRNA gene identified isolate L2C as Enterococcus faecalis.
AB - Bacterial isolates, which are able to degrade the azo textile dye Congo Red, have been isolated from Bantar Gebang Landfills-Bekasi and Citarum river, West Java. The initial screening using Zhou-Zimmerman medium which contains 50 ppm Congo Red yielded in 6 isolates (D2, I1, I4, L2B, L2C, and M) were able to degrade the dye. Selection for the best isolate using Bushnell-Haas medium showed that isolates L2C was able to degrade up to 98.5% of Congo Red within 3 days. The percentage of degradation was measured using Spectrophotometer at Λ 490 nm. Assessment of biodegradation profile of L2C at 500 ppm, 700 ppm and 1000 ppm of Congo Red showed that in 7 days, the isolates degraded the dye solution by 96.7%, 97.7% and 82.6%, respectively. The degrading rate of the re-isolated L2C cells at 1000 ppm of Congo Red increased up to 97.8% in 7 days when the cells were adapted into the Congo Red dye. Phytotoxicity test for germination using corn seed (Zea mays) showed that the toxicity of filtrate was slightly lower (50%) compared to the Congo Red 1000 ppm solution that is used as a control (67%). Molecular identification based on sequence 16S rRNA gene identified isolate L2C as Enterococcus faecalis.
UR - http://www.scopus.com/inward/record.url?scp=84984573558&partnerID=8YFLogxK
U2 - 10.1063/1.4946975
DO - 10.1063/1.4946975
M3 - Conference contribution
AN - SCOPUS:84984573558
T3 - AIP Conference Proceedings
BT - International Symposium on Current Progress in Mathematics and Sciences 2015, ISCPMS 2015
A2 - Mart, Terry
A2 - Triyono, Djoko
PB - American Institute of Physics Inc.
T2 - 1st International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2015
Y2 - 3 November 2015 through 4 November 2015
ER -