Isolation and characterization of potential bacteria with the ability to degrade Congo Red dye

Iman Santoso, Willy Purnomo, Haris Oktavianto, Wellyzar Sjamsuridzal, Windri Handayani, Sunardi

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Citation (Scopus)

Abstract

Bacterial isolates, which are able to degrade the azo textile dye Congo Red, have been isolated from Bantar Gebang Landfills-Bekasi and Citarum river, West Java. The initial screening using Zhou-Zimmerman medium which contains 50 ppm Congo Red yielded in 6 isolates (D2, I1, I4, L2B, L2C, and M) were able to degrade the dye. Selection for the best isolate using Bushnell-Haas medium showed that isolates L2C was able to degrade up to 98.5% of Congo Red within 3 days. The percentage of degradation was measured using Spectrophotometer at Λ 490 nm. Assessment of biodegradation profile of L2C at 500 ppm, 700 ppm and 1000 ppm of Congo Red showed that in 7 days, the isolates degraded the dye solution by 96.7%, 97.7% and 82.6%, respectively. The degrading rate of the re-isolated L2C cells at 1000 ppm of Congo Red increased up to 97.8% in 7 days when the cells were adapted into the Congo Red dye. Phytotoxicity test for germination using corn seed (Zea mays) showed that the toxicity of filtrate was slightly lower (50%) compared to the Congo Red 1000 ppm solution that is used as a control (67%). Molecular identification based on sequence 16S rRNA gene identified isolate L2C as Enterococcus faecalis.

Original languageEnglish
Title of host publicationInternational Symposium on Current Progress in Mathematics and Sciences 2015, ISCPMS 2015
Subtitle of host publicationProceedings of the 1st International Symposium on Current Progress in Mathematics and Sciences
EditorsTerry Mart, Djoko Triyono
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735413764
DOIs
Publication statusPublished - 19 Apr 2016
Event1st International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2015 - Depok, Indonesia
Duration: 3 Nov 20154 Nov 2015

Publication series

NameAIP Conference Proceedings
Volume1729
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference1st International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2015
Country/TerritoryIndonesia
CityDepok
Period3/11/154/11/15

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