TY - JOUR
T1 - Isolation and characterization of fungi from deteriorated old manuscripts from Banyumas, collection of Library of Universitas Indonesia
AU - Lintang, W.
AU - Susetyo-Salim, T.
AU - Oetari, A.
AU - Sjamsuridzal, W.
N1 - Funding Information:
This research was supported by Hibah Publikasi Terindeks Internasional (PUTI) Saintekes Universitas Indonesia Tahun Anggaran 2020 Number NKB-2390/UN2.RST/HKP.05.00/2020 to A.O. and Indonesia Endowment Fund for Education (LPDP RI) to W.L. The authors thank the Center of Excellence for Indigenous Biological Resources-Genome Studies, FMIPA Universitas Indonesia, for the use of facilities.
Publisher Copyright:
© Published under licence by IOP Publishing Ltd.
PY - 2021/12/16
Y1 - 2021/12/16
N2 - Fungi are the main cause of old manuscript deterioration since manuscripts provide carbon source and nutrient for fungal growth. Isolation of fungi from deteriorated old manuscripts from Banyumas was carried out and their morphology, xerophilic, and cellulolytic nature were investigated. Two deteriorated old dluwang manuscripts showed fungal spores, brown spots, and discoloured paper. Based on morphological characteristics, 31 fungal isolates belonged to five genera (Aspergillus Micheli, Cladosporium Link, Curvularia Boedijn, Penicillium Link, Ulocladium Preuss). These genera have been reported from deteriorated old manuscripts from several historical places in Indonesia. Xerophilic character was shown by 90% (28 isolates) as determined by growth in DG18 medium, which indicated the ability to grow in dry substrates such as old manuscripts. Cellulolytic character was shown by 93.5% (29 isolates) as determined by growth in dluwang paper and merang paper, which indicated that the papers were used as carbon sources and substrates. After 30 days-incubation, the dry weight loss of merang paper was 0.28-51.2%. Result from Scanning Electron Microscopy showed that the deterioration of merang paper were caused by the isolates as shown by the presence of fungal structures. These results showed that the fungal isolates were able to deteriorate old manuscripts from Banyumas, Indonesia.
AB - Fungi are the main cause of old manuscript deterioration since manuscripts provide carbon source and nutrient for fungal growth. Isolation of fungi from deteriorated old manuscripts from Banyumas was carried out and their morphology, xerophilic, and cellulolytic nature were investigated. Two deteriorated old dluwang manuscripts showed fungal spores, brown spots, and discoloured paper. Based on morphological characteristics, 31 fungal isolates belonged to five genera (Aspergillus Micheli, Cladosporium Link, Curvularia Boedijn, Penicillium Link, Ulocladium Preuss). These genera have been reported from deteriorated old manuscripts from several historical places in Indonesia. Xerophilic character was shown by 90% (28 isolates) as determined by growth in DG18 medium, which indicated the ability to grow in dry substrates such as old manuscripts. Cellulolytic character was shown by 93.5% (29 isolates) as determined by growth in dluwang paper and merang paper, which indicated that the papers were used as carbon sources and substrates. After 30 days-incubation, the dry weight loss of merang paper was 0.28-51.2%. Result from Scanning Electron Microscopy showed that the deterioration of merang paper were caused by the isolates as shown by the presence of fungal structures. These results showed that the fungal isolates were able to deteriorate old manuscripts from Banyumas, Indonesia.
KW - cellulolytic
KW - deterioration
KW - Dluwang paper
KW - SEM
KW - xerophilic
UR - http://www.scopus.com/inward/record.url?scp=85122305797&partnerID=8YFLogxK
U2 - 10.1088/1755-1315/948/1/012031
DO - 10.1088/1755-1315/948/1/012031
M3 - Conference article
AN - SCOPUS:85122305797
SN - 1755-1307
VL - 948
JO - IOP Conference Series: Earth and Environmental Science
JF - IOP Conference Series: Earth and Environmental Science
IS - 1
M1 - 012031
T2 - 4th International Conference on Biosciences, ICoBio 2021
Y2 - 11 August 2021 through 12 August 2021
ER -