Isolation and Anticancer Activity Assay of Stonefish (Synanceia horrida) Bioactive Stonustoxin

Anondho Wijanarko, Aulia Firdianna, Mikael Januardi Ginting, Kenny Lischer, Muhamad Sahlan, Heri Hermansyah

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Citation (Scopus)

Abstract

Breast cancer is a disease in which cells in the breast mutate and turn malignant, where the mutated cells spread to other parts of the body and develop into secondary cancer. In 2018, there were 58, 256 new cases of breast cancer. With improper handling, breast cancer can be lethal, where there are 22, 692 deaths in 2018 alone. One type of cancer with the most sufferers is estrogen, progesterone, and glucocorticoid receptors. Research on cancer treatment continues by looking for potential substances as new drugs. Toxins from animals that are cytolytic can trigger apoptosis in cancer cells. Some studies suggest that the lethal factor of the stonefish poison, Stonustoxin (SNTX), has cytolytic properties that damage cell membranes. This study discusses the stonefish (Synanceia horrida) venom as a breast anticancer agent. Stonefish poison was milked using a syringe, and the SNTX purification was carried out by the FPLC method using a 5 ml HiTrap Q H.P. substantial anion column. The percentage of inhibition of MCF-7 breast cancer cells will be measured using the MTT Assay method. As a result, MCF-7 breast cancer cells could be inhibited with IC50 values of 8.2771 μg/ml.

Original languageEnglish
Title of host publication6th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices
Subtitle of host publicationProceedings of the 6th International Symposium of Biomedical Engineering, ISBE 2021
EditorsSiti Fauziyah Rahman, Ahmad Zakiyuddin, Yudan Whulanza, Nurul Intan
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735443716
DOIs
Publication statusPublished - 16 Aug 2022
Event6th International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2021 - Depok, Virtual, Indonesia
Duration: 7 Jul 20218 Jul 2021

Publication series

NameAIP Conference Proceedings
Volume2537
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference6th International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2021
Country/TerritoryIndonesia
CityDepok, Virtual
Period7/07/218/07/21

Keywords

  • Breast cancer MCF-7
  • FPLC
  • MTT Assay
  • Stonustoxin

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