TY - GEN
T1 - Isolation and Anticancer Activity Assay of Stonefish (Synanceia horrida) Bioactive Stonustoxin
AU - Wijanarko, Anondho
AU - Firdianna, Aulia
AU - Ginting, Mikael Januardi
AU - Lischer, Kenny
AU - Sahlan, Muhamad
AU - Hermansyah, Heri
N1 - Funding Information:
This research and article's publication is supported by the Grant of Indexed International Publication (Publikasi Internasional Terindeks-PIT 9), funded by Universitas Indonesia No. NKB0043/UN2. R3.1/HKP.05.00/2019. The authors also thank all laboratory assistants at the ILRC Laboratory and staff at the Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia.
Publisher Copyright:
© 2022 American Institute of Physics Inc.. All rights reserved.
PY - 2022/8/16
Y1 - 2022/8/16
N2 - Breast cancer is a disease in which cells in the breast mutate and turn malignant, where the mutated cells spread to other parts of the body and develop into secondary cancer. In 2018, there were 58, 256 new cases of breast cancer. With improper handling, breast cancer can be lethal, where there are 22, 692 deaths in 2018 alone. One type of cancer with the most sufferers is estrogen, progesterone, and glucocorticoid receptors. Research on cancer treatment continues by looking for potential substances as new drugs. Toxins from animals that are cytolytic can trigger apoptosis in cancer cells. Some studies suggest that the lethal factor of the stonefish poison, Stonustoxin (SNTX), has cytolytic properties that damage cell membranes. This study discusses the stonefish (Synanceia horrida) venom as a breast anticancer agent. Stonefish poison was milked using a syringe, and the SNTX purification was carried out by the FPLC method using a 5 ml HiTrap Q H.P. substantial anion column. The percentage of inhibition of MCF-7 breast cancer cells will be measured using the MTT Assay method. As a result, MCF-7 breast cancer cells could be inhibited with IC50 values of 8.2771 μg/ml.
AB - Breast cancer is a disease in which cells in the breast mutate and turn malignant, where the mutated cells spread to other parts of the body and develop into secondary cancer. In 2018, there were 58, 256 new cases of breast cancer. With improper handling, breast cancer can be lethal, where there are 22, 692 deaths in 2018 alone. One type of cancer with the most sufferers is estrogen, progesterone, and glucocorticoid receptors. Research on cancer treatment continues by looking for potential substances as new drugs. Toxins from animals that are cytolytic can trigger apoptosis in cancer cells. Some studies suggest that the lethal factor of the stonefish poison, Stonustoxin (SNTX), has cytolytic properties that damage cell membranes. This study discusses the stonefish (Synanceia horrida) venom as a breast anticancer agent. Stonefish poison was milked using a syringe, and the SNTX purification was carried out by the FPLC method using a 5 ml HiTrap Q H.P. substantial anion column. The percentage of inhibition of MCF-7 breast cancer cells will be measured using the MTT Assay method. As a result, MCF-7 breast cancer cells could be inhibited with IC50 values of 8.2771 μg/ml.
KW - Breast cancer MCF-7
KW - FPLC
KW - MTT Assay
KW - Stonustoxin
UR - http://www.scopus.com/inward/record.url?scp=85138278318&partnerID=8YFLogxK
U2 - 10.1063/5.0098539
DO - 10.1063/5.0098539
M3 - Conference contribution
AN - SCOPUS:85138278318
T3 - AIP Conference Proceedings
BT - 6th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices
A2 - Rahman, Siti Fauziyah
A2 - Zakiyuddin, Ahmad
A2 - Whulanza, Yudan
A2 - Intan, Nurul
PB - American Institute of Physics Inc.
T2 - 6th International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2021
Y2 - 7 July 2021 through 8 July 2021
ER -