TY - JOUR
T1 - Is Tissue Oxygenation Causes Jaw Osteonecrosis in Osteoporotic Bone?
AU - Palupi, Anggraeny Putri Sekar
AU - Latief, Benny Sjariefsjah
AU - Priosoeryanto, Bambang Pontjo
AU - Suniarti, Dewi Fatma
AU - Dewi, Tri Isyani Tungga
N1 - Funding Information:
We would like to thank all Teaching Veterinary Hospital and Pathology Laboratory of Veterinary Medicine IPB University staff for their support during the experiment.
Publisher Copyright:
© 2022, Journal of International Dental and Medical Research. All Rights Reserved.
PY - 2022
Y1 - 2022
N2 - The pathogenesis of the occurrence of BRONJ until now cannot be determined with certainty. This paper investigates the effects of zoledronic acid (Z) on tissue oxygenation concerning the healing process of post-extraction tooth sockets. 24 female Sprague Dawley mice that had an ovariectomy were divided into three groups: a dose of 1x Z (n=8), a group of 6x Z (n=8), and a control group (n=8) who got 0.1mg/kg of Z through the intravena pathway. After six weeks, the extraction of the first molar teeth of the mandible. IHK and Elisa examinations were carried out on H+0 and H+14 post-extraction to observe HIF1-alpha, BMP2, and VWF expression concentrations. Statistical analysis results using the Kruskal-Wallis nonparametric test, the concentration of HIF1-alpha did not get a significant difference (p = 0.073). Likewise for the concentration of BMP2 (p = 0.123). As for the expression of also found no significant results (p = 0.076). The concentrations of HIF1-alpha and BMP2 tended to be higher in the Z group, although the results were not statistically significant. For VWF expression, the higher trend in the Z group was statistically insignificant. Therefore, caution is needed when interpreting the results of these three types of analysis with follow-up research dan clinical studies.
AB - The pathogenesis of the occurrence of BRONJ until now cannot be determined with certainty. This paper investigates the effects of zoledronic acid (Z) on tissue oxygenation concerning the healing process of post-extraction tooth sockets. 24 female Sprague Dawley mice that had an ovariectomy were divided into three groups: a dose of 1x Z (n=8), a group of 6x Z (n=8), and a control group (n=8) who got 0.1mg/kg of Z through the intravena pathway. After six weeks, the extraction of the first molar teeth of the mandible. IHK and Elisa examinations were carried out on H+0 and H+14 post-extraction to observe HIF1-alpha, BMP2, and VWF expression concentrations. Statistical analysis results using the Kruskal-Wallis nonparametric test, the concentration of HIF1-alpha did not get a significant difference (p = 0.073). Likewise for the concentration of BMP2 (p = 0.123). As for the expression of also found no significant results (p = 0.076). The concentrations of HIF1-alpha and BMP2 tended to be higher in the Z group, although the results were not statistically significant. For VWF expression, the higher trend in the Z group was statistically insignificant. Therefore, caution is needed when interpreting the results of these three types of analysis with follow-up research dan clinical studies.
KW - Bone morphogenetic proteins
KW - Hypoxia-inducible factor 1
KW - Osteonecrosis
KW - Zoledronic acid
UR - http://www.scopus.com/inward/record.url?scp=85138167376&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85138167376
SN - 1309-100X
VL - 15
SP - 1001
EP - 1005
JO - Journal of International Dental and Medical Research
JF - Journal of International Dental and Medical Research
IS - 3
ER -