TY - JOUR
T1 - Investigating the Effects of Hydro-alcoholic Urtica Dioica Extract and Retinoic Acid on Follicular Development
T2 - An Animal Study
AU - Jasim, Saade Abdalkareem
AU - Ali, Sana Abdul Jabbar
AU - Fadhil, Osama Q.
AU - Rakhmatova, Muqaddas Kh
AU - Kzar, Hamzah H.
AU - Margiana, Ria
AU - Al-Gazally, Moaed E.
AU - Jalil, Abduladheem Turki
AU - Mahmood, Zaid Hameed
AU - Aravindhan, Surendar
AU - Mustafa, Yasser Fakri
AU - Sultan, Mohammed Q.
N1 - Publisher Copyright:
© Iran University of Medical Sciences
PY - 2023
Y1 - 2023
N2 - Background: Urtica dioica (UD), as a natural antioxidant, has positive effects on oocyte maturation. This study aimed to investigate the effects of hydro-alcoholic UD extract and retinoic acid on follicular development in an in vitro fertilization (IVF) condition. Methods: A total of 40 female Wistar rats were randomly divided into 5 groups: group 1 received normal saline, group 2 was given 25 mg/kg retinoic acid, group 3 was administered with 100 mg/kg UD extract, group 4 was treated with retinoic acid plus UD extract, and group 5 received 10 mg/kg olive oil. The histomorphometric parameters were analyzed, including the number of follicles, follicular atrophy, fertilized oocytes, 2-cell embryos, dead embryos, and blastocysts. Results: Retinoic acid caused a significant increase in the primary, preantral, and atretic follicles and a substantial decrease in the corpus luteum compared with the control group (p<0.001). The number of preantral, antral follicles, and corpus luteum was significantly higher in group 3 compared with group 1 (p<0.001). Moreover, coadministration of UD plus retinoic acid (group 4) significantly reduced the atretic follicles (p<0.05).
AB - Background: Urtica dioica (UD), as a natural antioxidant, has positive effects on oocyte maturation. This study aimed to investigate the effects of hydro-alcoholic UD extract and retinoic acid on follicular development in an in vitro fertilization (IVF) condition. Methods: A total of 40 female Wistar rats were randomly divided into 5 groups: group 1 received normal saline, group 2 was given 25 mg/kg retinoic acid, group 3 was administered with 100 mg/kg UD extract, group 4 was treated with retinoic acid plus UD extract, and group 5 received 10 mg/kg olive oil. The histomorphometric parameters were analyzed, including the number of follicles, follicular atrophy, fertilized oocytes, 2-cell embryos, dead embryos, and blastocysts. Results: Retinoic acid caused a significant increase in the primary, preantral, and atretic follicles and a substantial decrease in the corpus luteum compared with the control group (p<0.001). The number of preantral, antral follicles, and corpus luteum was significantly higher in group 3 compared with group 1 (p<0.001). Moreover, coadministration of UD plus retinoic acid (group 4) significantly reduced the atretic follicles (p<0.05).
KW - Fertilization
KW - Histology
KW - Phytomedicine
KW - Retinoic Acid
KW - Urtica Dioica
UR - http://www.scopus.com/inward/record.url?scp=85150284979&partnerID=8YFLogxK
U2 - 10.47176/mjiri.37.1
DO - 10.47176/mjiri.37.1
M3 - Article
AN - SCOPUS:85150284979
SN - 1016-1430
VL - 37
JO - Medical Journal of the Islamic Republic of Iran
JF - Medical Journal of the Islamic Republic of Iran
IS - 1
M1 - 1
ER -