TY - GEN
T1 - Investigating different type of ovary cancer cell line for NK cell in vitro co-culture cytotoxic assay
AU - Pangjaya, Lady Feren
AU - Khaerunnisa, Sanya
AU - Mazfufah, Nuzli Fahdia
AU - Budiman, Retno Lestari
AU - Antarianto, Radiana Dhewayani
N1 - Publisher Copyright:
© 2021 Author(s).
PY - 2021/3/23
Y1 - 2021/3/23
N2 - Ovarian cancer is one of the gynecologic cancers with a high mortality rate and increased incidence. Surgery and therapies are used to treat ovarian cancer patients. Therapies such as chemotherapy and radiotherapy can cause resistance therefore immunotherapy using natural killer (NK) cells can be an alternative therapy. NK cells have shown an important role in anticancer activity because it is able to kill target cells without prior sensitization and can acquire immunology memory. However, NK cells as immunotherapy have low capacity and efficacy on infiltrating ovarian cancer's tumor microenvironment. To overcome these NK cells need to be induced with certain antigen resulting in enhanced NK cells. Enhanced NK cells display a higher cytotoxic activity that can be seen by co-culturing enhanced NK cells with ovarian cancer cell lines. In this mini-review, the selection of ovarian cancer cell lines was displayed to offer reliable consideration to obtain the expected research endpoint.
AB - Ovarian cancer is one of the gynecologic cancers with a high mortality rate and increased incidence. Surgery and therapies are used to treat ovarian cancer patients. Therapies such as chemotherapy and radiotherapy can cause resistance therefore immunotherapy using natural killer (NK) cells can be an alternative therapy. NK cells have shown an important role in anticancer activity because it is able to kill target cells without prior sensitization and can acquire immunology memory. However, NK cells as immunotherapy have low capacity and efficacy on infiltrating ovarian cancer's tumor microenvironment. To overcome these NK cells need to be induced with certain antigen resulting in enhanced NK cells. Enhanced NK cells display a higher cytotoxic activity that can be seen by co-culturing enhanced NK cells with ovarian cancer cell lines. In this mini-review, the selection of ovarian cancer cell lines was displayed to offer reliable consideration to obtain the expected research endpoint.
KW - co-culture
KW - cytotoxic assay
KW - NK cells
KW - ovarian cancer
KW - ovarian cancer cell lines
UR - http://www.scopus.com/inward/record.url?scp=85103511346&partnerID=8YFLogxK
U2 - 10.1063/5.0049155
DO - 10.1063/5.0049155
M3 - Conference contribution
AN - SCOPUS:85103511346
T3 - AIP Conference Proceedings
BT - 5th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices
A2 - Lischer, Kenny
A2 - Supriadi, Sugeng
A2 - Rahman, Siti Fauziyah
A2 - Whulanza, Yudan
PB - American Institute of Physics Inc.
T2 - 5th International Symposium of Biomedical Engineering, ISBE 2020
Y2 - 28 July 2020 through 29 July 2020
ER -
