TY - JOUR
T1 - Intravenous Versus Intraarterial Transplantation of Human Umbilical Cord Blood Mononuclear Cells for Brain Ischemia in Rats
AU - Ramli, Yetty
AU - Alwahdy, Ahmad Sulaiman
AU - Kurniawan, Mohammad
AU - Juliandi, Berry
AU - Wuyung, Puspita Eka
AU - Bilianti Susanto, Yayi Dwina
N1 - Funding Information:
The authors would like to thank National Institute of Health Research and Development (Litbangkes), Indonesia, for providing animals laboratory; Department of Pathology Anatomy, Faculty of Medicine, Universitas Indonesia, for assistance in histological analysis; and Integrated Care Unit for Stem Cell Therapy, National Hospital Cipto Mangunkusumo, for preparing the cells. This work has been funded by a grant from Universitas Indonesia (Grant no : 1753/UN2.R12/PPM.00.00/2016 ) “Hibah Publikasi Internasional Terindeks Untuk Tugas Akhir Mahasiswa UI 2016.”
Funding Information:
The authors would like to thank National Institute of Health Research and Development (Litbangkes), Indonesia, for providing animals laboratory; Department of Pathology Anatomy, Faculty of Medicine, Universitas Indonesia, for assistance in histological analysis; and Integrated Care Unit for Stem Cell Therapy, National Hospital Cipto Mangunkusumo, for preparing the cells. This work has been funded by a grant from Universitas Indonesia (Grant no : 1753/UN2.R12/PPM.00.00/2016) “Hibah Publikasi Internasional Terindeks Untuk Tugas Akhir Mahasiswa UI 2016.”
Publisher Copyright:
© 2017 Institut Pertanian Bogor
PY - 2017
Y1 - 2017
N2 - Cerebral ischemia is among the most common type of stroke seen in patient. Regeneration of death neurons remains questionable. Human umbilical cord blood mononuclear cell (cbMNC) is one of the treatment options for ischemia stroke through their various advantages; availability, pluripotency, and immaturity. One group of healthy rats and three groups (n = 6 per group) of male Wistar rats undergone permanent middle cerebral artery occlusion (MCAO). Rats were allowed to recover for 7 days before intraarterial and intravenous injection of 1 × 106 cells/kg of human cbMNC. Behavioral tests were performed before the MCAO, 1 week after MCAO, and at 3, 9, and 14 days after cbMNC injection. Brain infarct area and neurons in hippocampus were evaluated. Spontaneous activity was much significantly improved compared with the placebo group (p < 0.05). Comparing the neuron cells in hippocampus, intraarterial and intravenous have more changes in neurons morphology. No effect of cbMNC implantation in decreasing infarct area. Safety of xenogenic was confirmed by this study when the dosage of 1 × 106 cells/kg was used and showed their beneficial effects.
AB - Cerebral ischemia is among the most common type of stroke seen in patient. Regeneration of death neurons remains questionable. Human umbilical cord blood mononuclear cell (cbMNC) is one of the treatment options for ischemia stroke through their various advantages; availability, pluripotency, and immaturity. One group of healthy rats and three groups (n = 6 per group) of male Wistar rats undergone permanent middle cerebral artery occlusion (MCAO). Rats were allowed to recover for 7 days before intraarterial and intravenous injection of 1 × 106 cells/kg of human cbMNC. Behavioral tests were performed before the MCAO, 1 week after MCAO, and at 3, 9, and 14 days after cbMNC injection. Brain infarct area and neurons in hippocampus were evaluated. Spontaneous activity was much significantly improved compared with the placebo group (p < 0.05). Comparing the neuron cells in hippocampus, intraarterial and intravenous have more changes in neurons morphology. No effect of cbMNC implantation in decreasing infarct area. Safety of xenogenic was confirmed by this study when the dosage of 1 × 106 cells/kg was used and showed their beneficial effects.
KW - human umbilical cord blood
KW - middle cerebral artery occlusion
KW - monofilament
KW - mononuclear cells
KW - rats
UR - http://www.scopus.com/inward/record.url?scp=85039773699&partnerID=8YFLogxK
U2 - 10.1016/j.hjb.2017.11.002
DO - 10.1016/j.hjb.2017.11.002
M3 - Article
AN - SCOPUS:85039773699
SN - 1978-3019
VL - 24
SP - 187
EP - 194
JO - HAYATI Journal of Biosciences
JF - HAYATI Journal of Biosciences
IS - 4
ER -
