Interesterification of palm oil by using immobilized Candida rugosa lipase on Fe 3 O 4 -polydopamine nanoparticles

Sadwika Najmi Kautsari, Sri Handayani, Sumi Hudiyono Pws

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

4 Citations (Scopus)

Abstract

In this research, Candida rugosa lipase was used to produce fatty acid alkyl ester by interesterification reaction of palm oil with ethyl acetate or propyl acetate. The covalent immobilization of lipase on Fe 3 O 4 -polydopamine nanoparticles was prepared by coprecipitation methods. Magnetite (Fe 3 O 4 ) was used in lipase immobilization because of its binding efficiency and selective separation under a magnetic field. The immobilized lipase and Fe 3 O 4 -polydopamine nanoparticles were characterized using FTIR, XRD, and FESEM-EDS. Immobilized lipase has 84.8% of loading percentage and 3.67 U/mg of specific activity, while free lipase has 13.09 U/mg of specific activity. All sample showed the high composition percentage of oleic acid alkyl ester. Conversion percentage of fatty acid ethyl ester from free and immobilized were 17.48% and 9.93%. Conversion percentage of fatty acid propyl ester from free lipase and immobilized lipase were 11.57% and 9.88%. Immobilized lipase on Fe 3 O 4 -polydopamine nanoparticles could be used up to three times reaction and has a decrease of relative activity by 72.4%.

Original languageEnglish
Title of host publicationInternational Symposium on Current Progress in Mathematics and Sciences 2015, ISCPMS 2015
Subtitle of host publicationProceedings of the 1st International Symposium on Current Progress in Mathematics and Sciences
EditorsTerry Mart, Djoko Triyono
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735413764
DOIs
Publication statusPublished - 19 Apr 2016
Event1st International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2015 - Depok, Indonesia
Duration: 3 Nov 20154 Nov 2015

Publication series

NameAIP Conference Proceedings
Volume1729
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference1st International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2015
Country/TerritoryIndonesia
CityDepok
Period3/11/154/11/15

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