TY - JOUR
T1 - Inter-assay precision of clonogenic assays for radiosensitivity in cancer cell line A549
AU - Nuryadi, Endang
AU - Permata, Tiara Bunga Mayang
AU - Komatsu, Shuichiro
AU - Oike, Takahiro
AU - Nakano, Takashi
N1 - Funding Information:
This work was supported by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science,
Funding Information:
and Technology of Japan for programs for Leading Graduate Schools, Cultivating Global Leaders in Heavy Ion Therapeutics and Engineering. This work was also supported by Grants-in-Aid from the Japan Society for the Promotion of Science for KAKENHI.
Publisher Copyright:
© Nuryadi et al.
PY - 2018
Y1 - 2018
N2 - Clonogenic assays are the gold standard for determining radiosensitivity, which governs tumor response to radiation therapy. Although multiple studies of clonogenic assays on cancer cell lines have been published, the robustness of this technique has not been examined by comparative analysis of data from different studies. To address this issue, we investigated the inter-assay precision of clonogenic assays by analyzing in-house and published data on A549, a cell line frequently studied in this context. The coefficients of variation for SF2, the surviving fraction after 2 Gy irradiation, and D10, the radiation dose that reduces survival to 10%, were below 30% for both inhouse data obtained from 20 independent experiments performed under consistent experimental settings (i.e., radiation type, dose rate, and timing of cell seeding) and data collected from 192 publications using diverse experimental settings. Multivariate analyses of the published data revealed that timing of cell seeding significantly affected SF2. These data indicate that SF2 and D10 of clonogenic assay have acceptable inter-assay precision, and that timing of cell seeding influences the inter-assay precision of SF2. These results provide a rationale for combined analysis of published clonogenic assay data, which may help to discover robust biological properties associated with tumor radiosensitivity.
AB - Clonogenic assays are the gold standard for determining radiosensitivity, which governs tumor response to radiation therapy. Although multiple studies of clonogenic assays on cancer cell lines have been published, the robustness of this technique has not been examined by comparative analysis of data from different studies. To address this issue, we investigated the inter-assay precision of clonogenic assays by analyzing in-house and published data on A549, a cell line frequently studied in this context. The coefficients of variation for SF2, the surviving fraction after 2 Gy irradiation, and D10, the radiation dose that reduces survival to 10%, were below 30% for both inhouse data obtained from 20 independent experiments performed under consistent experimental settings (i.e., radiation type, dose rate, and timing of cell seeding) and data collected from 192 publications using diverse experimental settings. Multivariate analyses of the published data revealed that timing of cell seeding significantly affected SF2. These data indicate that SF2 and D10 of clonogenic assay have acceptable inter-assay precision, and that timing of cell seeding influences the inter-assay precision of SF2. These results provide a rationale for combined analysis of published clonogenic assay data, which may help to discover robust biological properties associated with tumor radiosensitivity.
KW - Cancer cell
KW - Clonogenic assay
KW - Meta-analysis
KW - Precision medicine
KW - Radiosensitivity
UR - http://www.scopus.com/inward/record.url?scp=85042716331&partnerID=8YFLogxK
U2 - 10.18632/oncotarget.24448
DO - 10.18632/oncotarget.24448
M3 - Article
AN - SCOPUS:85042716331
SN - 1949-2553
VL - 9
SP - 13706
EP - 13712
JO - Oncotarget
JF - Oncotarget
IS - 17
ER -