Insight into magnesium ions effect on chromosome banding and ultrastructure

Magnesium ion (Mg²⁺) plays a fundamental role in chromosome condensation which is important for genetic material segregation. Studies about the effects of Mg²⁺ on the overall chromosome structure have been reported. Nevertheless, its effects on the distribution of heterochromatin and euchromatin region have yet to be investigated. The aim of this study was to evaluate the effects of Mg²⁺ on the banding pattern and ultrastructure of the chromosome. Chromosome analysis was performed using the synchronized HeLa cells. The effect of Mg²⁺ was evaluated by subjecting the chromosomes to three different solutions, namely XBE5 (containing 5 mM Mg²⁺) as a control, XBE (0 mM Mg²⁺), and 1 mM EDTA as cations-chelator. Chromosome banding was carried out using the GTL-banding technique. The ultrastructure of the chromosomes treated with and without Mg²⁺ was further obtained using SEM. The results showed a condensed chromosome structure with a clear banding pattern when the chromosomes were treated with a buffer containing 5 mM Mg²⁺. In contrast, chromosomes treated with a buffer containing no Mg²⁺ and those treated with a cations-chelator showed an expanded and fibrous structure with the lower intensity of the banding pattern. Elongation of the chromosome caused by decondensation resulted in the band splitting. The different ultrastructure of the chromosomes treated with and without Mg²⁺ was obvious under SEM. The results of this study further emphasized the role of Mg²⁺ on chromosome structure and gave insights into Mg²⁺ effects on the banding distribution and ultrastructure of the chromosome.