TY - GEN
T1 - Inhibitory Activity of Uncaria Gambir Roxb Extract, Ethyl Acetate Fraction, and Catechin Isolate on Lipase
AU - Yunarto, Nanang
AU - Sulistyowati, Indah
AU - Reswandaru, Uud Nourma
AU - Elya, Berna
AU - Sauriasari, Rani
AU - Konadi, Laurentia
N1 - Funding Information:
The authors would like to thank Alifia Wardhani for her assistance in enzyme analysis. We also would like to thank Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan that funding the research.
Publisher Copyright:
© 2023 American Institute of Physics Inc.. All rights reserved.
PY - 2023/1/4
Y1 - 2023/1/4
N2 - Hypercholesterolemia is a main contributing risk factor to cardiovascular disease. Lipase inhibition lessens cholesterol biosynthesis in the liver. Statins are commonly used for hypercholesterolemia treatment. A lot of studies on natural products had been done to investigate potential inhibitors against lipase. Catechin as the essential compound in Uncaria gambir Roxb is conceivable for hypercholesterolemia treatment. This study aims to determine the inhibitory effect of extracts, ethyl acetate fraction, and catechin isolate of Uncaria gambir on lipase activity. Gambir leaf was extracted in water, the fractionation was caried out in ethyl acetate solvent while the isolation of catechin gambier was carried out in ethyl acetate-hexane as the solvent. Phytochemical analysis of extract and isolate was conducted by HPLC. The inhibitory activity of lipase enzymes was carried out using a lipase assay kit by ELISA as well as orlistat as the standard drug. This study has resulted in 68.32%, 92,26% and 96.74% catechin contained in extract, ethyl acetate fraction, and catechin isolate. The in-vitro inhibition assessment of lipase activity indicated that catechin isolate of Uncaria gambir was the most potential inhibitor of lipase (IC50 = 0.102 mg/mL), here in after ethyl acetate fraction supernatant (IC50 = 0.386 mg/mL), while extract and orlistat showed relatively less inhibition (IC50 = 0.680 mg/mL and IC50 = 0.645 mg/mL). Catechin isolate from Uncaria gambir showed strong inhibition of lipase activity. The outcome showed an equal correlation that the higher catechin, the higher inhibitory of lipase.
AB - Hypercholesterolemia is a main contributing risk factor to cardiovascular disease. Lipase inhibition lessens cholesterol biosynthesis in the liver. Statins are commonly used for hypercholesterolemia treatment. A lot of studies on natural products had been done to investigate potential inhibitors against lipase. Catechin as the essential compound in Uncaria gambir Roxb is conceivable for hypercholesterolemia treatment. This study aims to determine the inhibitory effect of extracts, ethyl acetate fraction, and catechin isolate of Uncaria gambir on lipase activity. Gambir leaf was extracted in water, the fractionation was caried out in ethyl acetate solvent while the isolation of catechin gambier was carried out in ethyl acetate-hexane as the solvent. Phytochemical analysis of extract and isolate was conducted by HPLC. The inhibitory activity of lipase enzymes was carried out using a lipase assay kit by ELISA as well as orlistat as the standard drug. This study has resulted in 68.32%, 92,26% and 96.74% catechin contained in extract, ethyl acetate fraction, and catechin isolate. The in-vitro inhibition assessment of lipase activity indicated that catechin isolate of Uncaria gambir was the most potential inhibitor of lipase (IC50 = 0.102 mg/mL), here in after ethyl acetate fraction supernatant (IC50 = 0.386 mg/mL), while extract and orlistat showed relatively less inhibition (IC50 = 0.680 mg/mL and IC50 = 0.645 mg/mL). Catechin isolate from Uncaria gambir showed strong inhibition of lipase activity. The outcome showed an equal correlation that the higher catechin, the higher inhibitory of lipase.
UR - http://www.scopus.com/inward/record.url?scp=85146522493&partnerID=8YFLogxK
U2 - 10.1063/5.0118363
DO - 10.1063/5.0118363
M3 - Conference contribution
AN - SCOPUS:85146522493
T3 - AIP Conference Proceedings
BT - Proceedings of the 8th International Symposium of Innovative Bioproduction Indonesia on Biotechnology and Bioengineering 2021
A2 - Harmoko, Rikno
A2 - Nuryana, Isa
A2 - Izzati, Fauzia Nurul
A2 - Sawono, Ki Ageng
A2 - Khaerunnisa, Isyana
A2 - Rahmawati, Siti Irma
A2 - Bayu, Asep
A2 - Martin, Andri Fadillah
A2 - Hertati, Ai
A2 - Subandiyah, Siti
A2 - Sawitri, Widhi Dyah
PB - American Institute of Physics Inc.
T2 - 8th International Symposium of Innovative Bioproduction Indonesia on Biotechnology and Bioengineering: Global Platform on Biodiversity and Biotechnology, ISIBio 2021
Y2 - 15 November 2021 through 16 November 2021
ER -
