Inhibition of HgCl2-induced mitogen-activated protein kinase activation by LL-Z1640-2 in CCRF-CEM cells

Masato Matsuoka; Bambang Wispriyono; Yoshihisa Iryo; Hideki Igisu; Tsutomu Sugiura

doi:10.1016/S0014-2999(00)00834-7

Inhibition of HgCl₂-induced mitogen-activated protein kinase activation by LL-Z1640-2 in CCRF-CEM cells

Masato Matsuoka, Bambang Wispriyono, Yoshihisa Iryo, Hideki Igisu, Tsutomu Sugiura

Department of Environmental Health Studies

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Exposure of HgCl₂ to CCRF-CEM human lymphoblastoid cells induced phosphorylation of mitogen-activated protein kinases (MAPKs); extracellular signal-regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK) and p38. LL-Z1640-2, a macrocyclic nonaketide, inhibited HgCl₂-induced JNK phosphorylation at 5-100 ng/ml. It also inhibited phosphorylation of ERK and p38 but only at 100 ng/ml. The same doses of radicicol did not suppress MAPKs activation. LL-Z1640-2 (at 100 ng/ml) inhibited HgCl₂-induced JNK phosphorylation in NIH 3T3 fibroblasts but not in LLC-PK₁ renal epithelial cells. Thus, LL-Z1640-2 is a potent inhibitor of HgCl₂-induced MAPKs activation, especially that of JNK, in CCRF-CEM cells. Copyright (C) 2000 Elsevier Science B.V.

Original language	English
Pages (from-to)	155-158
Number of pages	4
Journal	European Journal of Pharmacology
Volume	409
Issue number	2
DOIs	https://doi.org/10.1016/S0014-2999(00)00834-7
Publication status	Published - 8 Dec 2000

Keywords

HgCl
LL-Z1640-2
Mitogen-activated protein kinases
Radicicol
c-Jun N-terminal kinase

Access to Document

10.1016/S0014-2999(00)00834-7

Cite this

@article{03707d849a654b1682150baeb375f880,

title = "Inhibition of HgCl2-induced mitogen-activated protein kinase activation by LL-Z1640-2 in CCRF-CEM cells",

abstract = "Exposure of HgCl2 to CCRF-CEM human lymphoblastoid cells induced phosphorylation of mitogen-activated protein kinases (MAPKs); extracellular signal-regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK) and p38. LL-Z1640-2, a macrocyclic nonaketide, inhibited HgCl2-induced JNK phosphorylation at 5-100 ng/ml. It also inhibited phosphorylation of ERK and p38 but only at 100 ng/ml. The same doses of radicicol did not suppress MAPKs activation. LL-Z1640-2 (at 100 ng/ml) inhibited HgCl2-induced JNK phosphorylation in NIH 3T3 fibroblasts but not in LLC-PK1 renal epithelial cells. Thus, LL-Z1640-2 is a potent inhibitor of HgCl2-induced MAPKs activation, especially that of JNK, in CCRF-CEM cells. Copyright (C) 2000 Elsevier Science B.V.",

keywords = "HgCl, LL-Z1640-2, Mitogen-activated protein kinases, Radicicol, c-Jun N-terminal kinase",

author = "Masato Matsuoka and Bambang Wispriyono and Yoshihisa Iryo and Hideki Igisu and Tsutomu Sugiura",

note = "Funding Information: We gratefully acknowledge receipt of LL-Z1640-2 from Drs. Kenji Takehana and Tsuyoshi Kobayashi (Pharmaceutical Research Laboratories, Ajinomoto, Kawasaki, Japan). This work was supported in part by a fund from the Health Science Center Foundation, and a Grant-in-Aid for Scientific Research (C) from the Ministry of Education, Science, Sports and Culture, Japan.",

year = "2000",

month = dec,

day = "8",

doi = "10.1016/S0014-2999(00)00834-7",

language = "English",

volume = "409",

pages = "155--158",

journal = "European Journal of Pharmacology",

issn = "0014-2999",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - Inhibition of HgCl2-induced mitogen-activated protein kinase activation by LL-Z1640-2 in CCRF-CEM cells

AU - Matsuoka, Masato

AU - Wispriyono, Bambang

AU - Iryo, Yoshihisa

AU - Igisu, Hideki

AU - Sugiura, Tsutomu

N1 - Funding Information: We gratefully acknowledge receipt of LL-Z1640-2 from Drs. Kenji Takehana and Tsuyoshi Kobayashi (Pharmaceutical Research Laboratories, Ajinomoto, Kawasaki, Japan). This work was supported in part by a fund from the Health Science Center Foundation, and a Grant-in-Aid for Scientific Research (C) from the Ministry of Education, Science, Sports and Culture, Japan.

PY - 2000/12/8

Y1 - 2000/12/8

N2 - Exposure of HgCl2 to CCRF-CEM human lymphoblastoid cells induced phosphorylation of mitogen-activated protein kinases (MAPKs); extracellular signal-regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK) and p38. LL-Z1640-2, a macrocyclic nonaketide, inhibited HgCl2-induced JNK phosphorylation at 5-100 ng/ml. It also inhibited phosphorylation of ERK and p38 but only at 100 ng/ml. The same doses of radicicol did not suppress MAPKs activation. LL-Z1640-2 (at 100 ng/ml) inhibited HgCl2-induced JNK phosphorylation in NIH 3T3 fibroblasts but not in LLC-PK1 renal epithelial cells. Thus, LL-Z1640-2 is a potent inhibitor of HgCl2-induced MAPKs activation, especially that of JNK, in CCRF-CEM cells. Copyright (C) 2000 Elsevier Science B.V.

AB - Exposure of HgCl2 to CCRF-CEM human lymphoblastoid cells induced phosphorylation of mitogen-activated protein kinases (MAPKs); extracellular signal-regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK) and p38. LL-Z1640-2, a macrocyclic nonaketide, inhibited HgCl2-induced JNK phosphorylation at 5-100 ng/ml. It also inhibited phosphorylation of ERK and p38 but only at 100 ng/ml. The same doses of radicicol did not suppress MAPKs activation. LL-Z1640-2 (at 100 ng/ml) inhibited HgCl2-induced JNK phosphorylation in NIH 3T3 fibroblasts but not in LLC-PK1 renal epithelial cells. Thus, LL-Z1640-2 is a potent inhibitor of HgCl2-induced MAPKs activation, especially that of JNK, in CCRF-CEM cells. Copyright (C) 2000 Elsevier Science B.V.

KW - HgCl

KW - LL-Z1640-2

KW - Mitogen-activated protein kinases

KW - Radicicol

KW - c-Jun N-terminal kinase

UR - http://www.scopus.com/inward/record.url?scp=0034623719&partnerID=8YFLogxK

U2 - 10.1016/S0014-2999(00)00834-7

DO - 10.1016/S0014-2999(00)00834-7

M3 - Article

C2 - 11104828

AN - SCOPUS:0034623719

SN - 0014-2999

VL - 409

SP - 155

EP - 158

JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

IS - 2

ER -

Inhibition of HgCl₂-induced mitogen-activated protein kinase activation by LL-Z1640-2 in CCRF-CEM cells

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this