TY - JOUR
T1 - Inhibition of HgCl2-induced mitogen-activated protein kinase activation by LL-Z1640-2 in CCRF-CEM cells
AU - Matsuoka, Masato
AU - Wispriyono, Bambang
AU - Iryo, Yoshihisa
AU - Igisu, Hideki
AU - Sugiura, Tsutomu
N1 - Funding Information:
We gratefully acknowledge receipt of LL-Z1640-2 from Drs. Kenji Takehana and Tsuyoshi Kobayashi (Pharmaceutical Research Laboratories, Ajinomoto, Kawasaki, Japan). This work was supported in part by a fund from the Health Science Center Foundation, and a Grant-in-Aid for Scientific Research (C) from the Ministry of Education, Science, Sports and Culture, Japan.
PY - 2000/12/8
Y1 - 2000/12/8
N2 - Exposure of HgCl2 to CCRF-CEM human lymphoblastoid cells induced phosphorylation of mitogen-activated protein kinases (MAPKs); extracellular signal-regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK) and p38. LL-Z1640-2, a macrocyclic nonaketide, inhibited HgCl2-induced JNK phosphorylation at 5-100 ng/ml. It also inhibited phosphorylation of ERK and p38 but only at 100 ng/ml. The same doses of radicicol did not suppress MAPKs activation. LL-Z1640-2 (at 100 ng/ml) inhibited HgCl2-induced JNK phosphorylation in NIH 3T3 fibroblasts but not in LLC-PK1 renal epithelial cells. Thus, LL-Z1640-2 is a potent inhibitor of HgCl2-induced MAPKs activation, especially that of JNK, in CCRF-CEM cells. Copyright (C) 2000 Elsevier Science B.V.
AB - Exposure of HgCl2 to CCRF-CEM human lymphoblastoid cells induced phosphorylation of mitogen-activated protein kinases (MAPKs); extracellular signal-regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK) and p38. LL-Z1640-2, a macrocyclic nonaketide, inhibited HgCl2-induced JNK phosphorylation at 5-100 ng/ml. It also inhibited phosphorylation of ERK and p38 but only at 100 ng/ml. The same doses of radicicol did not suppress MAPKs activation. LL-Z1640-2 (at 100 ng/ml) inhibited HgCl2-induced JNK phosphorylation in NIH 3T3 fibroblasts but not in LLC-PK1 renal epithelial cells. Thus, LL-Z1640-2 is a potent inhibitor of HgCl2-induced MAPKs activation, especially that of JNK, in CCRF-CEM cells. Copyright (C) 2000 Elsevier Science B.V.
KW - HgCl
KW - LL-Z1640-2
KW - Mitogen-activated protein kinases
KW - Radicicol
KW - c-Jun N-terminal kinase
UR - http://www.scopus.com/inward/record.url?scp=0034623719&partnerID=8YFLogxK
U2 - 10.1016/S0014-2999(00)00834-7
DO - 10.1016/S0014-2999(00)00834-7
M3 - Article
C2 - 11104828
AN - SCOPUS:0034623719
SN - 0014-2999
VL - 409
SP - 155
EP - 158
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
IS - 2
ER -