Inhibition mechanism of Psidium guajava leaf to dengue virus replication in vitro

B. E. Dewi, I. Taufiqqurrachman, H. Desti, M. Sudiro, Fithriyah, M. Angelina

Research output: Contribution to journalConference articlepeer-review

1 Citation (Scopus)

Abstract

Dengue hemmorhagic fever is a disease caused by Dengue Virus (DENV) infection that carried by mosquito vector. The incidence of DHF in Indonesia (2013) is 41.25 cases per 100,000 population. However, until now there is no antiviral therapy for DHF. Last report explained the anti-Dengue activity in Psidium guajava leaves extract that have potency to inhibits the DENV infection with IC50 7.2 μg/mL and CC50 153.18 μg/mL. But, the mechanism still unknown. This research measure the percentage of viral inhibition in the DENV surface protein inhibition and receptor inhibition of 2 times IC50 extract using focus assay methods and followed by measure of cell viability using MTT assay. The results of focus assay for DENV surface protein inhibition is 58.24 ± 17.40% and in DENV receptor inhibition is 8.56 ± 6.29%. Then, the cell viability of attachment and receptor inhibition are 100.71 ± 4.72% and 100.96 ± 3.51% The bioactive compounds in the extract that acts as anti-Dengue are querectin and hyperoside. But, the action of quercetin is not on DENV surface protein or inhibits the DENV receptors and hyperoside acts to inhibits DENV receptor. This novelty suggest maybe there are other compounds in Psidium guajava leaves extract that acts as anti-Dengue.

Original languageEnglish
Article number012034
JournalIOP Conference Series: Earth and Environmental Science
Volume462
Issue number1
DOIs
Publication statusPublished - 15 Apr 2020
Event3rd International Conference on Natural Products and Bioresource Sciences 2019, ICONPROBIOS 2019 - BSD City, Tangerang, Indonesia
Duration: 23 Oct 201924 Oct 2019

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