TY - JOUR
T1 - Inhibition Activity of Angiotensin Converting Enzyme (ACE) and Determination of Total Phenolic and Flavonoid Compound from Bitter Melon Leaves (Momordica charantia L.)
AU - Lestari, Putri
AU - Katrin, null
AU - Rissyelly, null
AU - Mahayasih, Putu Gita Maya Widyaswari
N1 - Publisher Copyright:
© 2017 Phcog.Net.
PY - 2017
Y1 - 2017
N2 - Introduction: Hypertension is a leading problem in Indonesia, because of the high prevalence and the long term effect. Bitter melon leaves (Momordica charantia L.) has been traditionally utilized to treat hypertension, yet not many studies explained the antihypertension effect of this plant. Methods: This study was conducted to test the benefit of bitter melon leaves as antihypertension using angiotensin converting enzyme inhibition activity in 80% ethanolic extract and its fractions (n-hexane, ethyl acetate, and n-butanol) using ACE kit-WST (Dojindo, Japan). Then the antihypertension activity was associated with the phenolic content which was expressed in gallic acid equivalent (GAE) and total flavonoid in quercetin equivalent (QE). Results: Result of the study showed that the IC50 value obtained from the ethanolic extract to inhibit ACE activity was 7.52 μg/mL and the highest inhibition obtained in ethyl acetate fraction with IC50 value was 4,29 μg/mL. Phenolic total and flavonoid total determination also showed that the highest content obtained in ethyl acetate fraction with 18.752 mg GAE/gr extract and 8.310 mg QE/gr extract, respectively. Conclusion: According to the study, it could be concluded that bitter melon (Momordica charantia L.) leaves provide inhibition activity against Angiotensin Converting Enzyme (ACE) and chemical compounds that were expected to play an active role in inhibiting ACE were flavonoid and tannin.
AB - Introduction: Hypertension is a leading problem in Indonesia, because of the high prevalence and the long term effect. Bitter melon leaves (Momordica charantia L.) has been traditionally utilized to treat hypertension, yet not many studies explained the antihypertension effect of this plant. Methods: This study was conducted to test the benefit of bitter melon leaves as antihypertension using angiotensin converting enzyme inhibition activity in 80% ethanolic extract and its fractions (n-hexane, ethyl acetate, and n-butanol) using ACE kit-WST (Dojindo, Japan). Then the antihypertension activity was associated with the phenolic content which was expressed in gallic acid equivalent (GAE) and total flavonoid in quercetin equivalent (QE). Results: Result of the study showed that the IC50 value obtained from the ethanolic extract to inhibit ACE activity was 7.52 μg/mL and the highest inhibition obtained in ethyl acetate fraction with IC50 value was 4,29 μg/mL. Phenolic total and flavonoid total determination also showed that the highest content obtained in ethyl acetate fraction with 18.752 mg GAE/gr extract and 8.310 mg QE/gr extract, respectively. Conclusion: According to the study, it could be concluded that bitter melon (Momordica charantia L.) leaves provide inhibition activity against Angiotensin Converting Enzyme (ACE) and chemical compounds that were expected to play an active role in inhibiting ACE were flavonoid and tannin.
KW - Angiotensin converting enzyme
KW - Antihypertension
KW - Momordica charantia l.
KW - Total flavonoid
KW - Total phenolic
UR - http://www.scopus.com/inward/record.url?scp=85018504405&partnerID=8YFLogxK
U2 - 10.5530/pj.2017.2.43
DO - 10.5530/pj.2017.2.43
M3 - Article
AN - SCOPUS:85018504405
SN - 0975-3575
VL - 9
SP - 252
EP - 256
JO - Pharmacognosy Journal
JF - Pharmacognosy Journal
IS - 2
ER -