Influence of Lactose Concentration on Garut Sheep Oocyte Nuclear Visualization after Maturation

Research on the potential of lactose against post-maturation events of oocyte nuclear swollen of garut sheep (Ovis aries L.) has been carried out. The purpose of this study was to analyze the ability of lactose and to find the best concentration in visualizing the swollen oocyte nuclear after maturation. A total of 143 oocytes with A and B qualities were saturated in TCM-199 medium with the addition of Bovine Serum Albumin (BSA). Oocytes with nuclear status reached Metaphase II (M-II) as a result of in vitro maturation, then treated with various concentrations of lactose to see the nuclear swollen event. Oocyte nuclear swollen was observed using Hoechst & PI stain under fluorescence microscope. The results showed that the difference between concentrations by Kruskal-Wallis test (P > 0.05) and 3 % lactose concentration presented the highest percentage of oocyte nuclear swollen events in post-maturation oocytes (58.22 %). Post-maturation oocyte nuclear swollen events at 3% lactose concentration had a significant difference compared to 0 %, 1 %, and 5 % lactose concentrations. Lactose has the ability to swell the nuclear of garut sheep oocytes and 3 % lactose concentration is the best concentration capable of causing post-maturation of Garut oocyte nuclear swollen events.