Influence of inducers, Tween 80, and agitation on the enhancing decolorization of black liquor by Trametes versicolor F200

Black liquor wastewater was generated from bioethanol pre-treatment process. The combination of coagulant-flocculants, Poly Aluminium Chloride (PAC) and Anionic Polyacrylamides 3 % has able to reduce COD concentration 99.8 %. After coagulation-flocculation process, this wastewater was treated by Trametes versicolor F200. The aim of this study was to determine the decolourisation of coagulated black liquor by T. versicolor F200 and the effects of inducers, Tween 80, and agitation to enhance the decolourisation of coagulated black liquor. In addition to the decolourisation rate, COD concentration and enzyme activity were also measured in this study. Further, the characterization of lignin from black liquor was measured by LC-MS to determine the molecular weight of lignin before and after treatment by T. versicolor F200. The result showed that the optimum condition for obtaining the highest decolorization of coagulated black liquor were 97.56 % with addition of 2mM CuSO₄, 2mM MnSO₄, 2 % Tween 80 and agitation 150 rpm. The ranges of decolourisation during that condition were (97.56 - 97.89) %. Further, the decreasing molecular weight (m/z) of lignin during degradation process (539 to 325) showed that lignin compound can be degraded into smaller compound by using T. versicolor F200.