Inertial focusing and passive micro-mixing techniques for rare cells capturing microfluidic platform

Manisha Phadke; Sebastian Shaner; Shreyas Shah; Ygnacio Rodriguez; Denni Wibowo; Yudan Whulanza; Peter Teriete; Jeff Allen; Sam Kassegne

doi:10.1063/1.5023971

Inertial focusing and passive micro-mixing techniques for rare cells capturing microfluidic platform

Manisha Phadke, Sebastian Shaner, Shreyas Shah, Ygnacio Rodriguez, Denni Wibowo, Yudan Whulanza, Peter Teriete, Jeff Allen, Sam Kassegne

Department of Mechanical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

7 Citations (Scopus)

Abstract

Isolation and capture of rare cells continues to be a daunting task that is still looking for an innovative and efficient method. While a variety of approaches have been suggested over the past several years, immunocapturing in a microfluidic platform carries a substantial promise as shown by recent published works. In this paper, we introduced a combination of inertial focusing and passive micro-mixing through 3D chevron-type features in a microchannel to induce chaotic mixing within antibody-coated microchannels and, ultimately, promote rare cell capture. The device introduced in this work contains curved microchannels that consist of a series of staggered chevron grooves. The curved channels enable inertial focusing while the chevron grooves allow for chaotic mixing. The microfluidics platform microfabricated through soft lithography has a polydimethylsiloxane (PDMS) foundation and was thinly coated with an alginate hydrogel derivatized with streptavidin. We submitted that our qualitative and quantitative results demonstrated the potentials in advancements in rare cell isolation through this integration of two techniques.

Original language	English
Title of host publication	2nd Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, and Medical Devices
Subtitle of host publication	Proceedings of the International Symposium of Biomedical Engineering, ISBE 2017
Editors	Radon Dhelika, Yudan Whulanza, Ghiska Ramahdita, Praswasti P.D.K. Wulan
Publisher	American Institute of Physics Inc.
ISBN (Electronic)	9780735416253
DOIs	https://doi.org/10.1063/1.5023971
Publication status	Published - 13 Feb 2018
Event	2nd Biomedical Engineering's Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2017 - Bali, Indonesia Duration: 25 Jul 2017 → 26 Jul 2017

Publication series

Name	AIP Conference Proceedings
Volume	1933
ISSN (Print)	0094-243X
ISSN (Electronic)	1551-7616

Conference

Conference	2nd Biomedical Engineering's Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2017
Country/Territory	Indonesia
City	Bali
Period	25/07/17 → 26/07/17

Keywords

Microfluidics
inertial focusing
microchannels
rare-cell capture

Access to Document

10.1063/1.5023971

Cite this

Phadke, M., Shaner, S., Shah, S., Rodriguez, Y., Wibowo, D., Whulanza, Y., Teriete, P., Allen, J., & Kassegne, S. (2018). Inertial focusing and passive micro-mixing techniques for rare cells capturing microfluidic platform. In R. Dhelika, Y. Whulanza, G. Ramahdita, & P. P. D. K. Wulan (Eds.), 2nd Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2017 Article 040001 (AIP Conference Proceedings; Vol. 1933). American Institute of Physics Inc.. https://doi.org/10.1063/1.5023971

Phadke, Manisha ; Shaner, Sebastian ; Shah, Shreyas et al. / Inertial focusing and passive micro-mixing techniques for rare cells capturing microfluidic platform. 2nd Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2017. editor / Radon Dhelika ; Yudan Whulanza ; Ghiska Ramahdita ; Praswasti P.D.K. Wulan. American Institute of Physics Inc., 2018. (AIP Conference Proceedings).

@inproceedings{f44d1b8be70b4fb69715f87b6c32fd04,

title = "Inertial focusing and passive micro-mixing techniques for rare cells capturing microfluidic platform",

abstract = "Isolation and capture of rare cells continues to be a daunting task that is still looking for an innovative and efficient method. While a variety of approaches have been suggested over the past several years, immunocapturing in a microfluidic platform carries a substantial promise as shown by recent published works. In this paper, we introduced a combination of inertial focusing and passive micro-mixing through 3D chevron-type features in a microchannel to induce chaotic mixing within antibody-coated microchannels and, ultimately, promote rare cell capture. The device introduced in this work contains curved microchannels that consist of a series of staggered chevron grooves. The curved channels enable inertial focusing while the chevron grooves allow for chaotic mixing. The microfluidics platform microfabricated through soft lithography has a polydimethylsiloxane (PDMS) foundation and was thinly coated with an alginate hydrogel derivatized with streptavidin. We submitted that our qualitative and quantitative results demonstrated the potentials in advancements in rare cell isolation through this integration of two techniques.",

keywords = "Microfluidics, inertial focusing, microchannels, rare-cell capture",

author = "Manisha Phadke and Sebastian Shaner and Shreyas Shah and Ygnacio Rodriguez and Denni Wibowo and Yudan Whulanza and Peter Teriete and Jeff Allen and Sam Kassegne",

note = "Publisher Copyright: {\textcopyright} 2018 Author(s). Published by AIP Publishing.; 2nd Biomedical Engineering's Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2017 ; Conference date: 25-07-2017 Through 26-07-2017",

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Phadke, M, Shaner, S, Shah, S, Rodriguez, Y, Wibowo, D, Whulanza, Y, Teriete, P, Allen, J & Kassegne, S 2018, Inertial focusing and passive micro-mixing techniques for rare cells capturing microfluidic platform. in R Dhelika, Y Whulanza, G Ramahdita & PPDK Wulan (eds), 2nd Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2017., 040001, AIP Conference Proceedings, vol. 1933, American Institute of Physics Inc., 2nd Biomedical Engineering's Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2017, Bali, Indonesia, 25/07/17. https://doi.org/10.1063/1.5023971

Inertial focusing and passive micro-mixing techniques for rare cells capturing microfluidic platform. / Phadke, Manisha; Shaner, Sebastian; Shah, Shreyas et al.
2nd Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2017. ed. / Radon Dhelika; Yudan Whulanza; Ghiska Ramahdita; Praswasti P.D.K. Wulan. American Institute of Physics Inc., 2018. 040001 (AIP Conference Proceedings; Vol. 1933).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AU - Phadke, Manisha

AU - Shaner, Sebastian

AU - Shah, Shreyas

AU - Rodriguez, Ygnacio

AU - Wibowo, Denni

AU - Whulanza, Yudan

AU - Teriete, Peter

AU - Allen, Jeff

AU - Kassegne, Sam

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N2 - Isolation and capture of rare cells continues to be a daunting task that is still looking for an innovative and efficient method. While a variety of approaches have been suggested over the past several years, immunocapturing in a microfluidic platform carries a substantial promise as shown by recent published works. In this paper, we introduced a combination of inertial focusing and passive micro-mixing through 3D chevron-type features in a microchannel to induce chaotic mixing within antibody-coated microchannels and, ultimately, promote rare cell capture. The device introduced in this work contains curved microchannels that consist of a series of staggered chevron grooves. The curved channels enable inertial focusing while the chevron grooves allow for chaotic mixing. The microfluidics platform microfabricated through soft lithography has a polydimethylsiloxane (PDMS) foundation and was thinly coated with an alginate hydrogel derivatized with streptavidin. We submitted that our qualitative and quantitative results demonstrated the potentials in advancements in rare cell isolation through this integration of two techniques.

AB - Isolation and capture of rare cells continues to be a daunting task that is still looking for an innovative and efficient method. While a variety of approaches have been suggested over the past several years, immunocapturing in a microfluidic platform carries a substantial promise as shown by recent published works. In this paper, we introduced a combination of inertial focusing and passive micro-mixing through 3D chevron-type features in a microchannel to induce chaotic mixing within antibody-coated microchannels and, ultimately, promote rare cell capture. The device introduced in this work contains curved microchannels that consist of a series of staggered chevron grooves. The curved channels enable inertial focusing while the chevron grooves allow for chaotic mixing. The microfluidics platform microfabricated through soft lithography has a polydimethylsiloxane (PDMS) foundation and was thinly coated with an alginate hydrogel derivatized with streptavidin. We submitted that our qualitative and quantitative results demonstrated the potentials in advancements in rare cell isolation through this integration of two techniques.

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Phadke M, Shaner S, Shah S, Rodriguez Y, Wibowo D, Whulanza Y et al. Inertial focusing and passive micro-mixing techniques for rare cells capturing microfluidic platform. In Dhelika R, Whulanza Y, Ramahdita G, Wulan PPDK, editors, 2nd Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2017. American Institute of Physics Inc. 2018. 040001. (AIP Conference Proceedings). doi: 10.1063/1.5023971

Inertial focusing and passive micro-mixing techniques for rare cells capturing microfluidic platform

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