Induction of rat germ cell apoptosis by testosterone undecanoate and depot medroxyprogesterone acetate and correlation of apoptotic cells with sperm concentration

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Abstract

AIM: to investigate the safety and effectiveness of dose TU+DMPA hormones in developing potential male contraceptives.

METHODS: suppression of rat sperm concentration through increased apoptotic germ cells by in vivo administration of a long-acting androgen composed of a combination of testosterone undecanoate (TU) and depot medroxyprogesterone acetate (DMPA) was performed. Adult Sprague-Dawley rats received 2.5 mg TU every 6 week and 1.25 mg DMPA every 12 week for 60 week, a regimen known to rapidly reduce testosterone production by the testes and produce azoospermia within 12 week. Sperm concentration data were log transformed before analysis. Results are expressed as the mean±SEM. ANOVA, followed by post hoc test was used to determine differences across time and phase. T test was employed to determine differences between two groups.

RESULTS: apoptosis revealed significant increase in apoptotic germ cells (80% when rats were administered with TU+DMPA. Apoptotic germ cells can be found in several spermatogonia (20%), spermatocytes (30%), and spermatids (50%).

CONCLUSION: dose TU+DMPA hormones may be a safe and effective way to develop potential male contraceptives.

Original languageEnglish
Pages (from-to)32-37
Number of pages6
JournalActa medica Indonesiana
Volume45
Issue number1
Publication statusPublished - 1 Jan 2013

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