Indonesian honey protein isolation Apis dorsata dorsata and Tetragonula sp. as antibacterial and antioxidant agent

Muhamad Sahlan, Vina Damayanti, Nurul Azizah, Kazuaki Hakamada, Masafumi Yohda, Heri Hermansyah, Anondho Wijanarko, Etin Rohmatin

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

3 Citations (Scopus)

Abstract

Honey is a natural product that has many properties and been widely used for many theurapeutic purposes. Research on honey has been very rapid but not yet for Indonesia. Like local Indonesian honey Apis dorsata dorsata and Tetragonula sp. which has been widely consumed by the public but not yet known for certain efficacy of each content. The function of honey as antibacterial and antioxidant has not been specifically explained by the components contained in honey. Protein is one of the content of honey that turned out to have activity as an antibacterial and antioxidant in certain types of honey because of it antimicrobial peptide. Testing of honey activity as antibacterial and antioxidant through several stages including isolation, SDS-PAGE analysis, Bradford test, antibacterial activity test with well diffusion method and antioxidant activity test by DPPH method. Bacteria used were gram-positive bacteria Staphylococcus aureus and gram negative Escherichia coli. After some experiment finally got protein isolation method that is in the form of further concentration using Millipore membrane for honey Tetragonula sp. and membrane filtration dot blot for honey Apis dorsata dorsata. The Bradford assay showed that Apis dorsata dorsata honey contains protein <5 μg / ml, while honey Tetragonula sp. has a protein content of 97 μg / ml. The characteristic profile of molecular weight of the protein showed honey Tetragonula sp. has 3 protein bands composed of 52, 96 - 61,9 kDa, 63,35 - 65,92 kDa and 86,16 - 91,4 kDa, whereas Apis dorsata dorsata honey has 5 protein bands consisting of 45,2 - 46,6 kDa, 50,2 - 50,9 kDa, 62,5 - 62,9 kDa, 73,1 - 73,9 kDa, 83,9 - 86,9 kDa. Isolate honey protein Apis dorsata dorsata has no antioxidant and antibacterial activity (Staphylococcus aureus and Escherichia coli), whereas honey protein isolates Tetragonula sp. has antibacterial activity against Escherichia coli.

Original languageEnglish
Title of host publication2nd Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, and Medical Devices
Subtitle of host publicationProceedings of the International Symposium of Biomedical Engineering, ISBE 2017
EditorsRadon Dhelika, Yudan Whulanza, Ghiska Ramahdita, Praswasti P.D.K. Wulan
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735416253
DOIs
Publication statusPublished - 13 Feb 2018
Event2nd Biomedical Engineering's Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2017 - Bali, Indonesia
Duration: 25 Jul 201726 Jul 2017

Publication series

NameAIP Conference Proceedings
Volume1933
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference2nd Biomedical Engineering's Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2017
CountryIndonesia
CityBali
Period25/07/1726/07/17

Keywords

  • Antibacterial
  • Antioxidant
  • Apis dorsata dorsata
  • Honey
  • Isolation
  • Protein
  • Tetragonula sp.

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