In vivo study of 8-OHdG as a biomarker DNA damage by combining the exposure of nonyl phenol and copper using ELISA technique

Nonylphenol (NP) is an environmental contaminants produced by alkylphenol ethoxylates which is non-ionic surfactant. NP has recently been found in foods derived from antioxidant hydrolysis of tris nonylphenyl phosphite which is used as a heat stabilizer in the manufacture of polymer food packaging materials, such as styrene, vinyl polymer, rubber and polyolefins. Copper (Cu) is a redox metal which in the normal dose is an essential mineral that needed by the body, but excess Cu will cause damage in some organs. Both NP and Cu can produce OH radicals in the body, which means that OH radicals will potentially attack DNA and thus form a biomarker of DNA damage such as 8-OHdG. This study of DNA adducts of 8-OHdG formation due to NP as a xenobiotic and Cu as metals. In this research, the eight-month-old Sprague Dawly rats weighing 200 -300 g were randomly divided into three groups (Control, Cu, NP, and NP + Cu, n = 5). The tested rats were orally treated every 24 h for 28 days. Finally, the collected plasma was tested using ELISA technique to study the 8-OHdG content. Based on the results, it was shown that both NP and Cu metals could trigger the formation of 8-OHdG. The highest concentration of 8-OHdG formed was the exposure group of NP + Cu in day 14 with a concentration of 1.3412 ng/mL.