In Vivo antiplatelet activity aggregation assay of bromelain fractionate by ethanol from extract pineapple core (Ananas comosus [l.] merr.)

F. F. Musfiroh, Siswati Setiasih, Sri Handayani, Sumi Hudiyono Pws, N. M. Ilyas

Research output: Contribution to journalConference articlepeer-review

11 Citations (Scopus)

Abstract

Processed fruit from pineapple is one of largest commodities tropical fruit production in Indonesia and will bring the waste from the skin and core. This study aims to isolate bromelain from the pineapple core (Ananas comusus) are purified by fractionation using ethanol and continued by activity test as an antiplatelets agent by in vivo method using white mice male ddy type with acetosal as positive control. Fractionation of crude enzyme bromelain with ethanol produces highest specific activity on ethanol 30-60% fraction (fraction 2) 3.107 Unit/mg and the protein content 61.25 mg with the degree of purity of 155 times compared to crude enzyme. Antiplatelet aggregation tests from in vivo method shows that faction of bromelain with doses 70 μg/KgBW, 140 μg/KgBW, and 210 μg/KgBW can increase a meaningful bleeding time. The highest percentage of increase shown by the isolate at a dose of 210 μg/KgBW in the amount of 515.10 ± 182.23%, when compared to aspirin (231.20 ± 140.66), the value is relatively higher.

Original languageEnglish
Article number012017
JournalIOP Conference Series: Materials Science and Engineering
Volume299
Issue number1
DOIs
Publication statusPublished - 7 Feb 2018
Event1st International Conference on Chemistry and Material Science, IC2MS 2017 - Malang, East Java, Indonesia
Duration: 4 Nov 20175 Nov 2017

Keywords

  • Ananas comosus
  • antiplatelet
  • bromelain
  • in vivo
  • pineapple core
  • purification

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