TY - JOUR
T1 - In vivo antimammary tumor effects of soybean extract with targeted lunasin (ET-Lun)
AU - Rusdi, Numlil Khaira
AU - Purwaningsih, Erni Hernawati
AU - Hestiantoro, Andon
AU - Elya, Berna
AU - Kusmardi, Kusmardi
N1 - Funding Information:
Thank you to Directorate of Research & Development, Ministry of Research, Technology, and Higher Education Repulic of I ndonesia / National Agency for Research for the funds provided (PTUPT contract number NKB-318/UN2.RST/HKP.05.00/2020).
Publisher Copyright:
© 2021 Phcogj.Com.
PY - 2021/9
Y1 - 2021/9
N2 - Background/Objective: Lunasin is a peptide, consist of 44 amino acids which have anti-cancer, antioxidant, and anti-inflammatory activity. The price of commercial Lunasin was very expensive due to the high cost of lunasin synthesis and the lack of methods to obtain the pure lunasin weight from plant sources, involving time-consuming analytical instruments. To overcome these problems, the soybean extract with targeted Lunasin (ET-Lun) was made. The aim of this study was to investigate anti-cancer properties of ET-Lun in breast cancer models in vivo. Methods: Effect of ET-Lun was evaluated in 7,12-Dimetilbenz[a]antrasen (DMBA) induced breast cancer rat model. Tumor Mass, volume, and number were measured. The expression of HER2 and EGFR from each treatment group in DMBA-induced rat was evaluated using immunohistochemistry. Results: The results shown that ET-Lun could reduced tumor volume (p=0,021). ET-Lun decreased EGFR expression compared to negative control DMBA (p=0,012). Conclusions: These results indicated that the ET-Lun has anti-breast cancer activity in vivo.
AB - Background/Objective: Lunasin is a peptide, consist of 44 amino acids which have anti-cancer, antioxidant, and anti-inflammatory activity. The price of commercial Lunasin was very expensive due to the high cost of lunasin synthesis and the lack of methods to obtain the pure lunasin weight from plant sources, involving time-consuming analytical instruments. To overcome these problems, the soybean extract with targeted Lunasin (ET-Lun) was made. The aim of this study was to investigate anti-cancer properties of ET-Lun in breast cancer models in vivo. Methods: Effect of ET-Lun was evaluated in 7,12-Dimetilbenz[a]antrasen (DMBA) induced breast cancer rat model. Tumor Mass, volume, and number were measured. The expression of HER2 and EGFR from each treatment group in DMBA-induced rat was evaluated using immunohistochemistry. Results: The results shown that ET-Lun could reduced tumor volume (p=0,021). ET-Lun decreased EGFR expression compared to negative control DMBA (p=0,012). Conclusions: These results indicated that the ET-Lun has anti-breast cancer activity in vivo.
KW - Breast cancer
KW - EGFR
KW - HER2
KW - In-vivo
KW - Soybean
UR - http://www.scopus.com/inward/record.url?scp=85115297490&partnerID=8YFLogxK
U2 - 10.5530/pj.2021.13.160
DO - 10.5530/pj.2021.13.160
M3 - Article
AN - SCOPUS:85115297490
SN - 0975-3575
VL - 13
SP - 1269
EP - 1276
JO - Pharmacognosy Journal
JF - Pharmacognosy Journal
IS - 5
ER -