In vitro study of DNA adduct 8-OHDG formation from 2'-deoxyguanosine-5'-monophosphate with benzene through Fenton reaction

Budiawan, R. Erlindah, Sri Handayani, I. C. Dani

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Carcinogenic compounds from air pollution such as benzene could tend to generate free radicals and lead to DNA-radicals interaction producing 8-hydroxy-2'-deoxyguanosine (8-OHdG) as a biomarker of oxidative DNA damage. This research was conducted by reacting DNA base 2'-deoxyguanosine-5'-monophosphate (dGMP) with benzene in the variation of temperature 37 °C and 60 °C, variation of pH 7.4 and 8.4 during 5 hours of incubation time, and in the variation of Fe(II) and H 2 O 2 as reagent of Fenton reaction. The result of adduct was analyzed by using HPLC reversed phase with UV detector at a wavelength 254 nm. In this research, the retention time of dGMP standard was measured at 7.3 minutes and 8-OHdG was measured at 9.0 minutes. The formation of 8-OHdG from dGMP and benzene interaction in addition of Fe(II) at pH 8.4 and temperature 60 °C were higher than their interaction in the condition of pH 7.4 and temperature 37 °C. The presence of hydrogen peroxide under incubation condition at pH 8.4 and at temperature 60 °C can trigger the higher formation of 8-OHdG compared to the incubation condition at pH 7.4 and temperature 37 °C.

Original languageEnglish
Title of host publicationInternational Symposium on Current Progress in Mathematics and Sciences 2016, ISCPMS 2016
Subtitle of host publicationProceedings of the 2nd International Symposium on Current Progress in Mathematics and Sciences 2016
EditorsKiki Ariyanti Sugeng, Djoko Triyono, Terry Mart
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735415362
DOIs
Publication statusPublished - 10 Jul 2017
Event2nd International Symposium on Current Progress in Mathematics and Sciences 2016, ISCPMS 2016 - Depok, Jawa Barat, Indonesia
Duration: 1 Nov 20162 Nov 2016

Publication series

NameAIP Conference Proceedings
Volume1862
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference2nd International Symposium on Current Progress in Mathematics and Sciences 2016, ISCPMS 2016
Country/TerritoryIndonesia
CityDepok, Jawa Barat
Period1/11/162/11/16

Keywords

  • 8-OHdG
  • DNA adduct
  • benzene
  • fenton reaction

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