TY - JOUR
T1 - In vitro study of DNA Adduct 8-OHdG Formation by using Bisphenol A in Calf Thymus DNA and 2'-Deoxyguanosine
AU - Budiawan,
AU - Dani, Intan Cahaya
AU - Bakri, Ridla
AU - Handayani, Sri
AU - Dewi, Evi Ratna
N1 - Publisher Copyright:
© Published under licence by IOP Publishing Ltd.
PY - 2018/2/7
Y1 - 2018/2/7
N2 - The in vitro study of DNA Adduct 8-OHdG Formation due to BisphenolA (BPA) as xenobiotics has been conducted by using calf thymus DNA and 2'deoxyguanosine. The method of study was conducted by incubating calf thymus DNA and 2'dG with compounds trigger to radicals in the variation of pH (7.4 and 8.4), temperature (37°C and 60°C), and BPA concentrations (2 ppm and 10 ppm). To represent the work of CYP 450 enzyme in metabolic process of xenobiotics in the body and the effect of metal presence to the formation of radicals that can lead to 8-OHdG formation, we used iron(II) solution and also fenton reagent (Fe(II) and H2O2). The DNA used has 1.8 purity ratio (checked at λ260/λ280 by using Spectrophotometry UV-Vis). The results by using HPLC method showed that BPA could interact with DNA and DNA base (represent as calf thymus and 2'dG) and potentially induced 8-OHdG formation. The presence of iron(II) metal and Fenton reagent also induced the higher 8-OHdG formation. The higher of pH, temperature and concentrations also lead to 8-OHdG formation (ranger between 4 - 70 ppb).
AB - The in vitro study of DNA Adduct 8-OHdG Formation due to BisphenolA (BPA) as xenobiotics has been conducted by using calf thymus DNA and 2'deoxyguanosine. The method of study was conducted by incubating calf thymus DNA and 2'dG with compounds trigger to radicals in the variation of pH (7.4 and 8.4), temperature (37°C and 60°C), and BPA concentrations (2 ppm and 10 ppm). To represent the work of CYP 450 enzyme in metabolic process of xenobiotics in the body and the effect of metal presence to the formation of radicals that can lead to 8-OHdG formation, we used iron(II) solution and also fenton reagent (Fe(II) and H2O2). The DNA used has 1.8 purity ratio (checked at λ260/λ280 by using Spectrophotometry UV-Vis). The results by using HPLC method showed that BPA could interact with DNA and DNA base (represent as calf thymus and 2'dG) and potentially induced 8-OHdG formation. The presence of iron(II) metal and Fenton reagent also induced the higher 8-OHdG formation. The higher of pH, temperature and concentrations also lead to 8-OHdG formation (ranger between 4 - 70 ppb).
UR - http://www.scopus.com/inward/record.url?scp=85046257012&partnerID=8YFLogxK
U2 - 10.1088/1757-899X/299/1/012024
DO - 10.1088/1757-899X/299/1/012024
M3 - Conference article
AN - SCOPUS:85046257012
SN - 1757-8981
VL - 299
JO - IOP Conference Series: Materials Science and Engineering
JF - IOP Conference Series: Materials Science and Engineering
IS - 1
M1 - 012024
T2 - 1st International Conference on Chemistry and Material Science, IC2MS 2017
Y2 - 4 November 2017 through 5 November 2017
ER -