TY - JOUR
T1 - In-Vitro Study of DNA Adduct 8-Hydroxy-2'-Deoxyguanosine Formation from 2'-Dexoyguanosine Exposure Against Benzo[a]pyrene and Ni(II) Compounds Through Fenton-Like Reactions
AU - Farozy, Ikko Haidar
AU - Handayani, Sri
AU - Budiawan,
AU - Dani, Intan Cahaya
N1 - Publisher Copyright:
© Published under licence by IOP Publishing Ltd.
PY - 2019/10/9
Y1 - 2019/10/9
N2 - This research was performed to study the process of 8-hydroxy-2'-deoxyguanosine (8-OHdG) adduct formation as biomarker that used for early cancer detection in humans. This in-vitro study was conducted by reacting 2'-deoxyguanosine as one of the bases of DNA against benzo[a]pyrene (B[a]P) as xenobiotic, Ni(II), and H2O2, to study the effects of 2'- deoxyguanosine (2'-dG) exposure against xenobiotic through Fenton-like reaction. The reaction was incubated at 37°C for 18 hours in a shaker incubator. The reactions were varied at pH 7.4 and 8.4. The reaction products were then analyzed by using reverse phase HPLC with UV-Vis detector. The mobile phase used was a mixture of phosphate buffer and methanol with composition of 90:10. It was observed that exposure of 2'-dG against B[a]P and H2O2 and also B[a]P, Ni(II), and H2O2 both produced 8-OHdG. The higher 8-OHdG yield was obtained from the exposure of 2'-dG against B[a]P, Ni(II), and H2O2 compared to exposure against B[a]P and H2O2, which can be attributed to stronger effects from Fenton-like reaction. It was also observed that at similar exposure conditions, the 8-OHdG formation was higher at pH 7.4 compared to pH 8.4.
AB - This research was performed to study the process of 8-hydroxy-2'-deoxyguanosine (8-OHdG) adduct formation as biomarker that used for early cancer detection in humans. This in-vitro study was conducted by reacting 2'-deoxyguanosine as one of the bases of DNA against benzo[a]pyrene (B[a]P) as xenobiotic, Ni(II), and H2O2, to study the effects of 2'- deoxyguanosine (2'-dG) exposure against xenobiotic through Fenton-like reaction. The reaction was incubated at 37°C for 18 hours in a shaker incubator. The reactions were varied at pH 7.4 and 8.4. The reaction products were then analyzed by using reverse phase HPLC with UV-Vis detector. The mobile phase used was a mixture of phosphate buffer and methanol with composition of 90:10. It was observed that exposure of 2'-dG against B[a]P and H2O2 and also B[a]P, Ni(II), and H2O2 both produced 8-OHdG. The higher 8-OHdG yield was obtained from the exposure of 2'-dG against B[a]P, Ni(II), and H2O2 compared to exposure against B[a]P and H2O2, which can be attributed to stronger effects from Fenton-like reaction. It was also observed that at similar exposure conditions, the 8-OHdG formation was higher at pH 7.4 compared to pH 8.4.
UR - http://www.scopus.com/inward/record.url?scp=85074747457&partnerID=8YFLogxK
U2 - 10.1088/1742-6596/1245/1/012039
DO - 10.1088/1742-6596/1245/1/012039
M3 - Conference article
AN - SCOPUS:85074747457
SN - 1742-6588
VL - 1245
JO - Journal of Physics: Conference Series
JF - Journal of Physics: Conference Series
IS - 1
M1 - 012039
T2 - 7th Biannual International Conference on Mathematics and Natural Sciences, ICMNS 2018
Y2 - 2 November 2018 through 3 November 2018
ER -