In vitro study of DNA adduct 8-hidroxy-2'-deoxyguanosine (8-OHdG) formation through Fenton-like reaction with butylated hydroxytoluene quinone (BHT quinone)

Budiawan, Sri Handayani, I. C. Dani, Ridla Bakri, S. Hannaa, F. Irawati

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

In this research, in vitro study of DNA adduct 8-hidroxy-2'-deoxyguanosine (8-OHdG) formation as biomarkers of DNA damage was conducted by reacting 2'-deoxyguanosine (2'dG) with BHT-Q through Fenton-like reaction (Cr(III) and H 2 O 2 ). The conditions of reaction were varied in pH (7.4 and pH 8.4), temperature (37°C and 60°C) and incubation time (7 and 12 hours). The 8-OHdG produced was analyzed by using a reverse phase HPLC with UV-Vis detector. The result showed that 8-OHdG produced from reaction between 2'-dG, BHT-Q, in the presence of Cr(III), and H 2 O 2 was the highest (Fenton-like reaction). While, at a temperature of 60°C for 12 hours incubation time at pH 7.4 and 8.4, DNA adduct 8-OHdG formation was detected at each of 9.145 ppb and 10.601 ppb.

Original languageEnglish
Title of host publicationProceedings of the 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
EditorsRatna Yuniati, Terry Mart, Ivandini T. Anggraningrum, Djoko Triyono, Kiki A. Sugeng
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735417410
DOIs
Publication statusPublished - 22 Oct 2018
Event3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017 - Bali, Indonesia
Duration: 26 Jul 201727 Jul 2017

Publication series

NameAIP Conference Proceedings
Volume2023
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
Country/TerritoryIndonesia
CityBali
Period26/07/1727/07/17

Keywords

  • 8-Hidroxy-2-Deoxyguanosine (8-OHdG)
  • BHT-Quinone
  • DNA adduct
  • HPLC

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