@inproceedings{87c8c74521254a3c8db743d21b6b5093,
title = "In vitro study of DNA adduct 8-hidroxy-2'-deoxyguanosine (8-OHdG) formation through Fenton-like reaction with butylated hydroxytoluene quinone (BHT quinone)",
abstract = " In this research, in vitro study of DNA adduct 8-hidroxy-2'-deoxyguanosine (8-OHdG) formation as biomarkers of DNA damage was conducted by reacting 2'-deoxyguanosine (2'dG) with BHT-Q through Fenton-like reaction (Cr(III) and H 2 O 2 ). The conditions of reaction were varied in pH (7.4 and pH 8.4), temperature (37°C and 60°C) and incubation time (7 and 12 hours). The 8-OHdG produced was analyzed by using a reverse phase HPLC with UV-Vis detector. The result showed that 8-OHdG produced from reaction between 2'-dG, BHT-Q, in the presence of Cr(III), and H 2 O 2 was the highest (Fenton-like reaction). While, at a temperature of 60°C for 12 hours incubation time at pH 7.4 and 8.4, DNA adduct 8-OHdG formation was detected at each of 9.145 ppb and 10.601 ppb. ",
keywords = "8-Hidroxy-2-Deoxyguanosine (8-OHdG), BHT-Quinone, DNA adduct, HPLC",
author = "Budiawan and Sri Handayani and Dani, {I. C.} and Ridla Bakri and S. Hannaa and F. Irawati",
year = "2018",
month = oct,
day = "22",
doi = "10.1063/1.5064057",
language = "English",
series = "AIP Conference Proceedings",
publisher = "American Institute of Physics Inc.",
editor = "Ratna Yuniati and Terry Mart and Anggraningrum, {Ivandini T.} and Djoko Triyono and Sugeng, {Kiki A.}",
booktitle = "Proceedings of the 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017",
note = "3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017 ; Conference date: 26-07-2017 Through 27-07-2017",
}