TY - JOUR
T1 - In Vitro Phytochemical, Antioxidant, and Antibacterial Evaluations of Various Extracts of Eleocharis dulcis (Burm.f.) Trin. ex Hensch
AU - Amalia, Alfi
AU - Nugraha, Media F.I.
AU - Sukenda, Sukenda
AU - Elya, Berna
N1 - Funding Information:
The funding for the research was provided by BARISTA National Research and Innovation Agency of Indonesia (BRIN) scholarship (ref num. 91/II/HK/2022) and ministry of health scholarship.
Publisher Copyright:
© 2023 Amalia et al.
PY - 2023/6/1
Y1 - 2023/6/1
N2 - Eleocharis dulcis Burm.f. Trin. ex Hensch, has been empirically used in Chinese folk medicine to treat cough, laryngitis, hepatitis, enteritis, hypertension, and pharyngitis. The study is aimed to compare the phytochemical components, antioxidant and antibacterial activity of hexane (H), ethyl acetat (EA), and methanol extracts (MeOH) extracts obtained by Ultrasonic Assisted Extraction (UAE) method. Total Phenolic Content (TPC) was determined by the Folin-Ciocalteu method and Total Flavonoid Content (TFC) by quercetin method. Antioxidants activity using the DPPH, ABTS, and FRAP method. The inhibition zone of antibacterial activity using the disc diffusion method and Minimum Inhibitory Concentration with microdilution method. Results of the extracts’ total phenolic and flavonoid contents showed that EA has a higher content of these phytochemicals. The EA also exhibited a higher zone of inhibition for each concentration against Aeromonas hydrophila (AHA), Aeromonas salmonicida (AS), and Streptococcus agalactiae (SA). The MIC of EA against AHA and AS was 6.25 mg/mL and 3.125 mg/mL, respectively. All extracts were effective against SA with MIC of 1.5625 mg/mL. Similarly, the EA extract showed better antibacterial activity against all the tested bacteria strains than the H and MeOH extracts. Also, the EA extract exhibited better antioxidant activity compared to others, with IC50 values of 52.830 ± 0.271 ppm, 16.923 ± 0.047 ppm and 198.504 ± 1.490 µmol/g in the DPPH, ABTS and FRAP assays, respectively. The study concludes that the EA extract of E. dulcis possesses better antibacterial and antioxidant activities.
AB - Eleocharis dulcis Burm.f. Trin. ex Hensch, has been empirically used in Chinese folk medicine to treat cough, laryngitis, hepatitis, enteritis, hypertension, and pharyngitis. The study is aimed to compare the phytochemical components, antioxidant and antibacterial activity of hexane (H), ethyl acetat (EA), and methanol extracts (MeOH) extracts obtained by Ultrasonic Assisted Extraction (UAE) method. Total Phenolic Content (TPC) was determined by the Folin-Ciocalteu method and Total Flavonoid Content (TFC) by quercetin method. Antioxidants activity using the DPPH, ABTS, and FRAP method. The inhibition zone of antibacterial activity using the disc diffusion method and Minimum Inhibitory Concentration with microdilution method. Results of the extracts’ total phenolic and flavonoid contents showed that EA has a higher content of these phytochemicals. The EA also exhibited a higher zone of inhibition for each concentration against Aeromonas hydrophila (AHA), Aeromonas salmonicida (AS), and Streptococcus agalactiae (SA). The MIC of EA against AHA and AS was 6.25 mg/mL and 3.125 mg/mL, respectively. All extracts were effective against SA with MIC of 1.5625 mg/mL. Similarly, the EA extract showed better antibacterial activity against all the tested bacteria strains than the H and MeOH extracts. Also, the EA extract exhibited better antioxidant activity compared to others, with IC50 values of 52.830 ± 0.271 ppm, 16.923 ± 0.047 ppm and 198.504 ± 1.490 µmol/g in the DPPH, ABTS and FRAP assays, respectively. The study concludes that the EA extract of E. dulcis possesses better antibacterial and antioxidant activities.
KW - Eleocharis dulcis; Ultrasonic Assisted Extraction
KW - Flavonoids Content
KW - Phenolics Content
UR - http://www.scopus.com/inward/record.url?scp=85163156101&partnerID=8YFLogxK
U2 - 10.26538/tjnpr/v7i5.11
DO - 10.26538/tjnpr/v7i5.11
M3 - Article
AN - SCOPUS:85163156101
SN - 2616-0684
VL - 7
SP - 2911
EP - 2918
JO - Tropical Journal of Natural Product Research
JF - Tropical Journal of Natural Product Research
IS - 5
ER -