In vitro cytotoxicity of the synthesized gallic acid derivatives (N-Alkyl Gallamide) against breast MCF-7 cancer cells

Maurin Marcelia; Ade Arsianti; Jilly Octaria Tagore Chan; STEv ANO JULIO Wijoyo; Fadilah Fadilah; Rista Putrianingsih; Norma Nur Azizah; Anton Bahtiar; Hiroki Tanimoto; Kiyomikakiuchi

doi:10.13005/ojc/340506

In vitro cytotoxicity of the synthesized gallic acid derivatives (N-Alkyl Gallamide) against breast MCF-7 cancer cells

Maurin Marcelia, Ade Arsianti, Jilly Octaria Tagore Chan, STEv ANO JULIO Wijoyo, Fadilah Fadilah, Rista Putrianingsih, Norma Nur Azizah, Anton Bahtiar, Hiroki Tanimoto, Kiyomikakiuchi

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3 Citations (Scopus)

Abstract

Gallic acid is a phenolic compound distributed in plants and fruits which has been reported to have cytotoxic effect on MCF-7 breast cancer cell line. In this research, we investigated in vitro cytotoxic effect of six synthesized compounds of gallic acid derivatives (N-alkyl gallamide), namely N-methyl gallamide (2); N-ethyl gallamide (3); N-butyl gallamide (4); N-sec-butyl gallamide (5); N-tert-butyl gallamide (6) and N-hexyl gallamide (7) against breast MCF-7 cells by MTT assay. Linear regression analysis is utilized to analyze data to regenerate IC₅₀ value. The results will be compared with gallic acid as an original compound and doxorubicin as a positive control. Among six synthesized compounds, N-tert-butyl gallamide (6) with IC₅₀ value of 2.1 µg/mL, and N-hexyl gallamide (7) with IC₅₀ value of 3.5µg/mL,showed the stronger cytotoxicity against breast MCF-7 cells compared to gallic acid and doxorubicin. Thus,N-tert-butyl gallamide (6) and N-hexyl gallamide (7) are potential to be further developed as a promising anti-breast cancer agents.

Original language	English
Pages (from-to)	2268-2272
Number of pages	5
Journal	Oriental Journal of Chemistry
Volume	34
Issue number	5
DOIs	https://doi.org/10.13005/ojc/340506
Publication status	Published - 2018

Keywords

Breast MCF-7 cells
Cytotoxicity
Gallic acid derivative
MTT assay
N-alkyl gallamide

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Marcelia, M., Arsianti, A., Chan, J. O. T., Wijoyo, STE. ANO. JULIO., Fadilah, F., Putrianingsih, R., Azizah, N. N., Bahtiar, A., Tanimoto, H., & Kiyomikakiuchi (2018). In vitro cytotoxicity of the synthesized gallic acid derivatives (N-Alkyl Gallamide) against breast MCF-7 cancer cells. Oriental Journal of Chemistry, 34(5), 2268-2272. https://doi.org/10.13005/ojc/340506

@article{ac17c4578f5c46c294dd41d707b593db,

title = "In vitro cytotoxicity of the synthesized gallic acid derivatives (N-Alkyl Gallamide) against breast MCF-7 cancer cells",

abstract = "Gallic acid is a phenolic compound distributed in plants and fruits which has been reported to have cytotoxic effect on MCF-7 breast cancer cell line. In this research, we investigated in vitro cytotoxic effect of six synthesized compounds of gallic acid derivatives (N-alkyl gallamide), namely N-methyl gallamide (2); N-ethyl gallamide (3); N-butyl gallamide (4); N-sec-butyl gallamide (5); N-tert-butyl gallamide (6) and N-hexyl gallamide (7) against breast MCF-7 cells by MTT assay. Linear regression analysis is utilized to analyze data to regenerate IC50 value. The results will be compared with gallic acid as an original compound and doxorubicin as a positive control. Among six synthesized compounds, N-tert-butyl gallamide (6) with IC50 value of 2.1 µg/mL, and N-hexyl gallamide (7) with IC50 value of 3.5µg/mL,showed the stronger cytotoxicity against breast MCF-7 cells compared to gallic acid and doxorubicin. Thus,N-tert-butyl gallamide (6) and N-hexyl gallamide (7) are potential to be further developed as a promising anti-breast cancer agents.",

keywords = "Breast MCF-7 cells, Cytotoxicity, Gallic acid derivative, MTT assay, N-alkyl gallamide",

author = "Maurin Marcelia and Ade Arsianti and Chan, {Jilly Octaria Tagore} and Wijoyo, {STEv ANO JULIO} and Fadilah Fadilah and Rista Putrianingsih and Azizah, {Norma Nur} and Anton Bahtiar and Hiroki Tanimoto and Kiyomikakiuchi",

note = "Funding Information: We sincerely thank to Directorate of Research and Community Engagement (DRPM) and Faculty of Medicine, Universitas Indonesia for the PITTA research grant. We also thank to Division of Materials Science, Nara Institute of Science and Technology (NAIST), Japan, for NAIST Global Collaborative Research Program. Funding Information: We sincerely thank to Directorate of Research and Community Engagement (DRPM) and Faculty of Medicine, Universitas Indonesia for the PITTA research grant.We also thank to Division of Materials Science, Nara Institute of Science and Technology (NAIST), Japan, for NAIST Global Collaborative Research Program. Publisher Copyright: {\textcopyright} Oriental Scientific Publishing Company. All rights reserved.",

year = "2018",

doi = "10.13005/ojc/340506",

language = "English",

volume = "34",

pages = "2268--2272",

journal = "Oriental Journal of Chemistry",

issn = "0970-020X",

publisher = "Bhopal, S.A. Iqbal",

number = "5",

}

TY - JOUR

T1 - In vitro cytotoxicity of the synthesized gallic acid derivatives (N-Alkyl Gallamide) against breast MCF-7 cancer cells

AU - Marcelia, Maurin

AU - Arsianti, Ade

AU - Chan, Jilly Octaria Tagore

AU - Wijoyo, STEv ANO JULIO

AU - Fadilah, Fadilah

AU - Putrianingsih, Rista

AU - Azizah, Norma Nur

AU - Bahtiar, Anton

AU - Tanimoto, Hiroki

AU - Kiyomikakiuchi,

N1 - Funding Information: We sincerely thank to Directorate of Research and Community Engagement (DRPM) and Faculty of Medicine, Universitas Indonesia for the PITTA research grant. We also thank to Division of Materials Science, Nara Institute of Science and Technology (NAIST), Japan, for NAIST Global Collaborative Research Program. Funding Information: We sincerely thank to Directorate of Research and Community Engagement (DRPM) and Faculty of Medicine, Universitas Indonesia for the PITTA research grant.We also thank to Division of Materials Science, Nara Institute of Science and Technology (NAIST), Japan, for NAIST Global Collaborative Research Program. Publisher Copyright: © Oriental Scientific Publishing Company. All rights reserved.

PY - 2018

Y1 - 2018

N2 - Gallic acid is a phenolic compound distributed in plants and fruits which has been reported to have cytotoxic effect on MCF-7 breast cancer cell line. In this research, we investigated in vitro cytotoxic effect of six synthesized compounds of gallic acid derivatives (N-alkyl gallamide), namely N-methyl gallamide (2); N-ethyl gallamide (3); N-butyl gallamide (4); N-sec-butyl gallamide (5); N-tert-butyl gallamide (6) and N-hexyl gallamide (7) against breast MCF-7 cells by MTT assay. Linear regression analysis is utilized to analyze data to regenerate IC50 value. The results will be compared with gallic acid as an original compound and doxorubicin as a positive control. Among six synthesized compounds, N-tert-butyl gallamide (6) with IC50 value of 2.1 µg/mL, and N-hexyl gallamide (7) with IC50 value of 3.5µg/mL,showed the stronger cytotoxicity against breast MCF-7 cells compared to gallic acid and doxorubicin. Thus,N-tert-butyl gallamide (6) and N-hexyl gallamide (7) are potential to be further developed as a promising anti-breast cancer agents.

AB - Gallic acid is a phenolic compound distributed in plants and fruits which has been reported to have cytotoxic effect on MCF-7 breast cancer cell line. In this research, we investigated in vitro cytotoxic effect of six synthesized compounds of gallic acid derivatives (N-alkyl gallamide), namely N-methyl gallamide (2); N-ethyl gallamide (3); N-butyl gallamide (4); N-sec-butyl gallamide (5); N-tert-butyl gallamide (6) and N-hexyl gallamide (7) against breast MCF-7 cells by MTT assay. Linear regression analysis is utilized to analyze data to regenerate IC50 value. The results will be compared with gallic acid as an original compound and doxorubicin as a positive control. Among six synthesized compounds, N-tert-butyl gallamide (6) with IC50 value of 2.1 µg/mL, and N-hexyl gallamide (7) with IC50 value of 3.5µg/mL,showed the stronger cytotoxicity against breast MCF-7 cells compared to gallic acid and doxorubicin. Thus,N-tert-butyl gallamide (6) and N-hexyl gallamide (7) are potential to be further developed as a promising anti-breast cancer agents.

KW - Breast MCF-7 cells

KW - Cytotoxicity

KW - Gallic acid derivative

KW - MTT assay

KW - N-alkyl gallamide

UR - http://www.scopus.com/inward/record.url?scp=85055886007&partnerID=8YFLogxK

U2 - 10.13005/ojc/340506

DO - 10.13005/ojc/340506

M3 - Article

AN - SCOPUS:85055886007

SN - 0970-020X

VL - 34

SP - 2268

EP - 2272

JO - Oriental Journal of Chemistry

JF - Oriental Journal of Chemistry

IS - 5

ER -

In vitro cytotoxicity of the synthesized gallic acid derivatives (N-Alkyl Gallamide) against breast MCF-7 cancer cells

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Keywords

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