In vitro cytotoxicity of the synthesized gallic acid derivatives (N-Alkyl Gallamide) against breast MCF-7 cancer cells

Maurin Marcelia, Ade Arsianti, Jilly Octaria Tagore Chan, STEv ANO JULIO Wijoyo, Fadilah, Rista Putrianingsih, Norma Nur Azizah, Anton Bahtiar, Hiroki Tanimoto, Kiyomikakiuchi

Research output: Contribution to journalArticle

Abstract

Gallic acid is a phenolic compound distributed in plants and fruits which has been reported to have cytotoxic effect on MCF-7 breast cancer cell line. In this research, we investigated in vitro cytotoxic effect of six synthesized compounds of gallic acid derivatives (N-alkyl gallamide), namely N-methyl gallamide (2); N-ethyl gallamide (3); N-butyl gallamide (4); N-sec-butyl gallamide (5); N-tert-butyl gallamide (6) and N-hexyl gallamide (7) against breast MCF-7 cells by MTT assay. Linear regression analysis is utilized to analyze data to regenerate IC50 value. The results will be compared with gallic acid as an original compound and doxorubicin as a positive control. Among six synthesized compounds, N-tert-butyl gallamide (6) with IC50 value of 2.1 µg/mL, and N-hexyl gallamide (7) with IC50 value of 3.5µg/mL,showed the stronger cytotoxicity against breast MCF-7 cells compared to gallic acid and doxorubicin. Thus,N-tert-butyl gallamide (6) and N-hexyl gallamide (7) are potential to be further developed as a promising anti-breast cancer agents.

Original languageEnglish
Pages (from-to)2268-2272
Number of pages5
JournalOriental Journal of Chemistry
Volume34
Issue number5
DOIs
Publication statusPublished - 1 Jan 2018

Keywords

  • Breast MCF-7 cells
  • Cytotoxicity
  • Gallic acid derivative
  • MTT assay
  • N-alkyl gallamide

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    Marcelia, M., Arsianti, A., Chan, J. O. T., Wijoyo, STE. ANO. JULIO., Fadilah, Putrianingsih, R., Azizah, N. N., Bahtiar, A., Tanimoto, H., & Kiyomikakiuchi (2018). In vitro cytotoxicity of the synthesized gallic acid derivatives (N-Alkyl Gallamide) against breast MCF-7 cancer cells. Oriental Journal of Chemistry, 34(5), 2268-2272. https://doi.org/10.13005/ojc/340506