In vitro bioassay of n-buthanol isolate of Acorus calamus L. on inhibitory of activity a-glucosidase

Compound existence at one particular plant extract ingredient that has ability to inhibitory α-glucosidase enzyme, give a chance to develop a natural medicine in the effort therapy of diabetic. This aim of study to see effectivity of n-buthanol isolate from plants Acorus calamus L. on inhibitory α -glucosidase enzyme. Extraction done with masceration method use methanol. The gummy methanol extract followed by partition using n-heksan, ethyl acetate and n-butanol. The nbuthanol extract isolate using column chromatography method with stationer phase is resin diaion hp 20 and eluent ethanol:Water in various concentration, isolation result is got 5 fraction. In each fraction was assayed for inhibitory activity on α-glucosidase enzyme. The potential inhibition effect was got in 5 ^th fraction and from purified process using column chromatography method with stationer phase is silica gel and eluent dichloromethane: methanol 95:5 was got ACB as active compound with IC ₅₀ 17.89 μg/ml. This result shows that plants Acorus calamus L. has a potency to develop upon which diabetes therapy.