TY - JOUR
T1 - In vitro assay of alpha-glucosidase inhibitor activities of three seagrasses from Banten Bay, Indonesia
AU - Widiyanto, Agung
AU - Anwar, Effionora
AU - Nurhayati, Tati
N1 - Publisher Copyright:
© 2018 Phcog.Net.
PY - 2018
Y1 - 2018
N2 - Introduction: Diabetes mellitus (DM) is an endocrine disorder characterized by high blood glucose levels. One of the approaches for treating DM is by regulating the postprandial blood glucose via inhibition of α-glucosidase enzymes. Seagrass is known as a plant containing bioactive compounds, especially for therapy antidiabetic.This research aims to evaluate the α-glucosidase inhibition activity of three species seagrasses (Enhalus acoroides, Thalassia hemprichii and Cymodocea rotundata) from the Banten Bay, Indonesia. Methods: The extracts of all parts (root, leaf, and stem) from three seagrasses were prepared with maceration method using 80% ethanol solvents. The α-glucosidase inhibitory activity was conducted by microplate reader at 400 nm using acarbose as a positive control. Furthermore, the kinetics of α-glucosidase inhibition, phytochemical screening, and total phenolics content were evaluated against extract which has the most potential α-glucosidase inhibition. Results: The result showed that the 80% ethanol extracts of Enhalus acoroides (IC50 values 168.15 ± 2.71 µg/mL) had the most potential α-glucosidase inhibitors activity compared with the positive control acarbose, Thalassia hemprichii and Cymodocea rotundata (IC50 values 197.27 ± 3.07 µg/mL, 425.86 ± 5.15 µg/mL and 429.28 ± 8.89 µg/mL). The kinetic type of inhibition against α-glucosidase was noncompetitive inhibition. The phytochemical compounds were phenols, flavonoid, terpenes, and tannin with the total phenolic content was 28.76 ± 2.46 mgGAE/g. Conclusion: Enhalus acoroides has the strongest inhibitor of α-glucosidase and can be further developed for DM therapy agents.
AB - Introduction: Diabetes mellitus (DM) is an endocrine disorder characterized by high blood glucose levels. One of the approaches for treating DM is by regulating the postprandial blood glucose via inhibition of α-glucosidase enzymes. Seagrass is known as a plant containing bioactive compounds, especially for therapy antidiabetic.This research aims to evaluate the α-glucosidase inhibition activity of three species seagrasses (Enhalus acoroides, Thalassia hemprichii and Cymodocea rotundata) from the Banten Bay, Indonesia. Methods: The extracts of all parts (root, leaf, and stem) from three seagrasses were prepared with maceration method using 80% ethanol solvents. The α-glucosidase inhibitory activity was conducted by microplate reader at 400 nm using acarbose as a positive control. Furthermore, the kinetics of α-glucosidase inhibition, phytochemical screening, and total phenolics content were evaluated against extract which has the most potential α-glucosidase inhibition. Results: The result showed that the 80% ethanol extracts of Enhalus acoroides (IC50 values 168.15 ± 2.71 µg/mL) had the most potential α-glucosidase inhibitors activity compared with the positive control acarbose, Thalassia hemprichii and Cymodocea rotundata (IC50 values 197.27 ± 3.07 µg/mL, 425.86 ± 5.15 µg/mL and 429.28 ± 8.89 µg/mL). The kinetic type of inhibition against α-glucosidase was noncompetitive inhibition. The phytochemical compounds were phenols, flavonoid, terpenes, and tannin with the total phenolic content was 28.76 ± 2.46 mgGAE/g. Conclusion: Enhalus acoroides has the strongest inhibitor of α-glucosidase and can be further developed for DM therapy agents.
KW - Cymodocea rotundata
KW - Diabetes mellitus
KW - Enhalus acoroides
KW - Phytochemical compound
KW - Thalassia hemprichii
UR - http://www.scopus.com/inward/record.url?scp=85051760037&partnerID=8YFLogxK
U2 - 10.5530/pj.2018.5.152
DO - 10.5530/pj.2018.5.152
M3 - Article
AN - SCOPUS:85051760037
SN - 0975-3575
VL - 10
SP - 907
EP - 910
JO - Pharmacognosy Journal
JF - Pharmacognosy Journal
IS - 5
ER -