In vitro antioxidant and cytotoxicity activity of extract and fraction Pyrrosia piloselloides (L) M.G price

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Abstract

The herbs of Pyrrosia piloselloides (L) M.G Price are well known as traditional medicine in Indonesia for breast cancer. This research was conducted to evaluate the of cytotoxic effect of P. piloselloides (L) M.G Price on MCF-7 Breast cancer cell and in vitro antioxidant activity. P. piloselloides (L) M.G Price herbs macerated successively using n-hexane, dichloromethane and metanol for result n-hexane extract (EHP), dichloromethane extract (EDCMP) and methanol extract (EMP). Cytotoxicity test using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Antioxidant activity test using the 1,1 diphenyl-2-picrylhydrazyl (DPPH). The results showed that EDCM has the potential as an antioxidant with the smallest IC50 value that is equal to 12.82 μg/mL and best cytotoxity with IC50 39.54 μg/mL. EDCMP fractionated using column chromatography with eluent mixture of n-hexane-ethyl acetate and ethyl acetatemethanol with increasing polarity. The results obtained by fractionation of six fractions (FI-FVI EDCMP). Antioxidant activity assay results showed that FIV EDCMP have the greatest antioxidant activity with IC50 value of 28.29 μg/mL. Cytotoxicity activity results that FVIEDCMP have the greatest with IC50 value 28.73 μg/mL. P. piloselloides (L) M.G Price potent for inhibit breast cancer and antioxidant.

Original languageEnglish
Pages (from-to)119-125
Number of pages7
JournalInternational Journal of PharmTech Research
Volume5
Issue number1
Publication statusPublished - 1 Jan 2013

Keywords

  • Antioxidant
  • Cytotoxixity
  • DPPH
  • MCF-7
  • MTT assay
  • Pyrrosia piloselloides (L) M.G price

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