TY - JOUR
T1 - In-vitro ANTICANCER ACTIVITY OF Saurauia vulcani LEAF EXTRACTS AGAINST HUMAN COLON WIDR AND HCT-116 CANCER CELLS
AU - Pasaribu, G.
AU - Hudiyono, S.
AU - Budianto, E.
AU - Cahyana, A. H.
AU - Pari, G.
N1 - Funding Information:
This research was funded by the PUTI Doktor-2020 project at the University of Indonesia. On this occasion, the authors wish to convey high appreciation, express sincere gratitude, and feel deeply indebted to the PUTI Doktor-2020 project for funding; and providing assistance as well as facilities in performing this research. Further appreciations are extended to all the authors and the involved parties for their comprehensive cooperation to accomplish this research ultimately.
Publisher Copyright:
© 2021, Rasayan Journal of Chemistry, c/o Dr. Pratima Sharma. All rights reserved.
PY - 2021
Y1 - 2021
N2 - Saurauia vulcani plants grow naturally in North Sumatra, Indonesia. This plant is commonly used as an antidiabetic drug and a cure for other digestive ailments. The objective of this study was to examine the phytochemical characteristics and anti-colorectal-cancer activity of the extract from leaves of Saurauia vulcani plants. The method employed was to test phytochemicals that included the presence/detection of alkaloids, saponins, flavonoids, triterpenoids/ steroids, tannins, and hydroquinone in the leaf extract. The anti-colorectal-cancer activity was tested in vitro using the MTT method, against WiDr and HCT 116 cancer cells, with the liquid fractions of n-hexane, ethyl acetate, and methanol solvents. Phytochemical analysis results showed that Saurauia vulcani leaf extract contained tannins, saponins, flavonoids, and terpenoids. Chemical component analysis of ethyl acetate fraction showed the presence of fatty acid groups. The n-hexane, ethyl acetate, and methanol fractions exhibited cytotoxicity against the WiDr cell line, with IC50 values of 456.19, 97.41 and 191.92 ppm, respectively. Meanwhile, the cytotoxicity activity of the leaf extract against the HCT cell lines was accomplished at IC50 values of 777.35, 568.53 and 529.39 ppm, correspondingly with n-hexane, ethyl acetate and methanol fractions, respectively. The leaf extract in ethyl acetate fraction was indicatively the best in efficaciously inhibiting the WiDr cells.
AB - Saurauia vulcani plants grow naturally in North Sumatra, Indonesia. This plant is commonly used as an antidiabetic drug and a cure for other digestive ailments. The objective of this study was to examine the phytochemical characteristics and anti-colorectal-cancer activity of the extract from leaves of Saurauia vulcani plants. The method employed was to test phytochemicals that included the presence/detection of alkaloids, saponins, flavonoids, triterpenoids/ steroids, tannins, and hydroquinone in the leaf extract. The anti-colorectal-cancer activity was tested in vitro using the MTT method, against WiDr and HCT 116 cancer cells, with the liquid fractions of n-hexane, ethyl acetate, and methanol solvents. Phytochemical analysis results showed that Saurauia vulcani leaf extract contained tannins, saponins, flavonoids, and terpenoids. Chemical component analysis of ethyl acetate fraction showed the presence of fatty acid groups. The n-hexane, ethyl acetate, and methanol fractions exhibited cytotoxicity against the WiDr cell line, with IC50 values of 456.19, 97.41 and 191.92 ppm, respectively. Meanwhile, the cytotoxicity activity of the leaf extract against the HCT cell lines was accomplished at IC50 values of 777.35, 568.53 and 529.39 ppm, correspondingly with n-hexane, ethyl acetate and methanol fractions, respectively. The leaf extract in ethyl acetate fraction was indicatively the best in efficaciously inhibiting the WiDr cells.
KW - Anti-cancer Activity
KW - Colorectal-cancer Cells
KW - Leaf Extract
KW - Plant
KW - Saurauia vulcani
UR - http://www.scopus.com/inward/record.url?scp=85129596944&partnerID=8YFLogxK
U2 - 10.31788/RJC.2021.1456449
DO - 10.31788/RJC.2021.1456449
M3 - Article
AN - SCOPUS:85129596944
SN - 0974-1496
VL - 2021
SP - 165
EP - 174
JO - Rasayan Journal of Chemistry
JF - Rasayan Journal of Chemistry
IS - Special Issue
ER -