TY - JOUR
T1 - Improving Enzyme-Assisted Extraction of Brazilin from Sappanwood (Caesalpinia Sappan L.) Extract by Fungal Cellulase
AU - Septiani, Dia
AU - Suryadi, Herman
AU - Mun’im, Abdul
N1 - Funding Information:
This research was supported by Penelitian Tesis Magister (3055) Grant Research by Ministry of Research, Technology, and Higher Education of the Republic of Indonesia.
Publisher Copyright:
© 2022 Phcogj.Com. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
PY - 2022/1
Y1 - 2022/1
N2 - Brazilin was one of phytoconstituent from sappanwood that widely used as textile-colouring agent and found in traditional concoction for therapeutic purposes. Due to obtain its maximum level in sappanwood (Caesalpinia sappan L.) extract, the extraction method of brazilin has been developed. Enzyme-assisted extraction (EAE) is one of green chemistry methods to achieve that outcome. This study aims to enhance brazilin level by optimize the EAE condition with fungi cellulase. The cellulase are produced by monoculture Aspergillus niger UICC371 in carboxymethyl cellulose submerged fermentation’s media. Sappanwood extracted with fungi cellulase through variation conditions: enzyme concentrations (2.0; 4,0; 6.0%); temperature (45, 50, 55℃); and time (1, 2, 3 hrs). The optimization are provided by response surface method-BoxBehnken design and brazilin level was carried out through High Performance Liquid Chromatography (HPLC) with asetonitril: 0,3% acetic acid in water (14.5: 85.5) as eluents. The study showed that cellulase from monoculture of Aspergillus niger UICC371 showed optimum condition of cellulase-EAE method at 6.0% concentration enzyme at 50℃ for 3 hours extraction time which provide an increase in brazilin level to 5.014% compare to reflux method.
AB - Brazilin was one of phytoconstituent from sappanwood that widely used as textile-colouring agent and found in traditional concoction for therapeutic purposes. Due to obtain its maximum level in sappanwood (Caesalpinia sappan L.) extract, the extraction method of brazilin has been developed. Enzyme-assisted extraction (EAE) is one of green chemistry methods to achieve that outcome. This study aims to enhance brazilin level by optimize the EAE condition with fungi cellulase. The cellulase are produced by monoculture Aspergillus niger UICC371 in carboxymethyl cellulose submerged fermentation’s media. Sappanwood extracted with fungi cellulase through variation conditions: enzyme concentrations (2.0; 4,0; 6.0%); temperature (45, 50, 55℃); and time (1, 2, 3 hrs). The optimization are provided by response surface method-BoxBehnken design and brazilin level was carried out through High Performance Liquid Chromatography (HPLC) with asetonitril: 0,3% acetic acid in water (14.5: 85.5) as eluents. The study showed that cellulase from monoculture of Aspergillus niger UICC371 showed optimum condition of cellulase-EAE method at 6.0% concentration enzyme at 50℃ for 3 hours extraction time which provide an increase in brazilin level to 5.014% compare to reflux method.
KW - Caesalpinia sappan L
KW - Cellulase
KW - Enzyme assisted extraction
KW - Fungi
KW - Response surface method
UR - http://www.scopus.com/inward/record.url?scp=85125699925&partnerID=8YFLogxK
U2 - 10.5530/pj.2022.14.4
DO - 10.5530/pj.2022.14.4
M3 - Article
AN - SCOPUS:85125699925
SN - 0975-3575
VL - 14
SP - 21
EP - 28
JO - Pharmacognosy Journal
JF - Pharmacognosy Journal
IS - 1
ER -