TY - JOUR
T1 - Implementation of 25-well culture plates for M. tuberculosis drug susceptibility testing in Indonesia
AU - Rosana, Yeva
AU - Sudiro, Tjahjani Mirawati
AU - Alisjahbana, Bachti
AU - Parwati, Ida
AU - van Crevel, Reinout
AU - van Soolingen, Dick
N1 - Publisher Copyright:
© 2005, Faculty of Medicine, Universitas Indonesia. All rights reserved.
PY - 2005/7/1
Y1 - 2005/7/1
N2 - At present, there is no standardized method for Mycobacterium tuberculosis drug susceptibility testing (DST) among laboratories in Indonesia. Since January 2001 to January 2004 we have tried to establish the method of 25-well culture plates with middlebrook’s media (Drug Susceptibility Culture Plate (DSCP) method) used by the Dutch Supranational Reference Laboratory at the Institute of Public Health and the Environment (RIVM), Bilthoven, Netherlands. Our experience showed that this method potentially gives better result as it can be very well standardized, faster and provides detailed MIC (Minimal Inhibitory Concentration) values. Data from 364 isolates that have been tested by DSCP method showed that resistance to INH, rifampicin, ethambutol, and streptomycin were 21.4%, 19.8%, 15.7%, and 16.5% respectively. Multidrug resistance were found in 13.2% isolates.
AB - At present, there is no standardized method for Mycobacterium tuberculosis drug susceptibility testing (DST) among laboratories in Indonesia. Since January 2001 to January 2004 we have tried to establish the method of 25-well culture plates with middlebrook’s media (Drug Susceptibility Culture Plate (DSCP) method) used by the Dutch Supranational Reference Laboratory at the Institute of Public Health and the Environment (RIVM), Bilthoven, Netherlands. Our experience showed that this method potentially gives better result as it can be very well standardized, faster and provides detailed MIC (Minimal Inhibitory Concentration) values. Data from 364 isolates that have been tested by DSCP method showed that resistance to INH, rifampicin, ethambutol, and streptomycin were 21.4%, 19.8%, 15.7%, and 16.5% respectively. Multidrug resistance were found in 13.2% isolates.
KW - DSCP method
KW - DST
KW - M. tuberculosis
UR - http://www.scopus.com/inward/record.url?scp=85008618993&partnerID=8YFLogxK
U2 - 10.13181/mji.v14i3.186
DO - 10.13181/mji.v14i3.186
M3 - Article
AN - SCOPUS:85008618993
SN - 0853-1773
VL - 14
SP - 142
EP - 146
JO - Medical Journal of Indonesia
JF - Medical Journal of Indonesia
IS - 3
ER -