Implementation DNA barcoding for genetic identification of cuscus from Ambon Island

E. N. Kusumaningrum, Abinawanto

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Citation (Scopus)

Abstract

Cuscus or cusu is one endemic marsupial that found in Indonesia. Based on The IUCN redlist, the population is least concern due to human activity such as land use, hunting, and animal trading. The aim of the study was to identify species cuscus in Ambon Island based on Cytochrome Oxydase subunit I (COI) gene. DNA samples were obtained from feces cuscus then amplified using PCR method. The sequencing results were analyzed using MEGA 7.0 version program and compared with other cuscus species obtained from Genbank. Construction of phylogeny tree was create using Neighbor Joining method with bootstrap 1000 times. The results showed that the species are suspected Spilocuscus maculatus and Phalanger vestitus.

Original languageEnglish
Title of host publicationProceedings of the 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
EditorsRatna Yuniati, Terry Mart, Ivandini T. Anggraningrum, Djoko Triyono, Kiki A. Sugeng
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735417410
DOIs
Publication statusPublished - 22 Oct 2018
Event3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017 - Bali, Indonesia
Duration: 26 Jul 201727 Jul 2017

Publication series

NameAIP Conference Proceedings
Volume2023
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
CountryIndonesia
CityBali
Period26/07/1727/07/17

Keywords

  • Cuscus
  • DNA Barcoding
  • PCR method

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