TY - JOUR
T1 - Impact of serum human chorionic gonadotropin and luteinizing hormone receptor expression to oocyte maturation rate
T2 - A study of controlled ovarian stimulation
AU - Nora, Hilwah
AU - Wiweko, Budi
AU - Muharam, R.
AU - Rajuddin,
AU - Wangge, Grace
AU - Hestiantoro, Andon
AU - Pratama, Gita
AU - Harzif, Achmad Kemal
AU - Zakirah, Sarah
N1 - Publisher Copyright:
© 2020 Journal of Human Reproductive Sciences | Published by Wolters Kluwer-Medknow.
PY - 2020/1/1
Y1 - 2020/1/1
N2 - Background: Few studies have assessed the impact of serum human chorionic gonadotropin (hCG) levels before oocyte retrieval and luteinizing hormone receptor (LHR) mRNA expression. Aims: The objective was to assess the correlations between serum hCG levels at 12-h posttrigger granulosa cell LHR mRNA expression during the in vitro fertilization (IVF) cycle with oocyte maturation rate and to determine the cutoff level of serum hCG at 12-h posttrigger. Settings and Design: A cross-sectional was conducted for this study at the IVF center of Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia. Materials and Methods: Recombinant follicle-stimulating hormone was used on day 2 of the menstrual cycle with multiple doses of a gonadotropin-releasing hormone antagonist. Recombinant hCG was used to trigger ovulation. At 12-h posttrigger, hCG serum levels were measured using an enzyme-linked immunosorbent assay. Statistical Analysis: Pearson's correlation coefficient was used to evaluate the correlation between oocyte maturation rates, serum hCG levels, and LHR mRNA levels. Cutoff values were determined using a receiver operating characteristic (ROC) curve. Results: Serum hCG levels were positively correlated (r = 0.467;P < 0.01), and LHR mRNA expression was weakly correlated (r = 0.073; P = 0.701) with oocyte maturation. The cutoff of serum hCG for a high maturation rate was 77 mIU/mL, with an area under the ROC curve of 0.765 (95% confidence interval: 0.598-0.939) andP < 0.001. Conclusion: Oocyte maturation is correlated with serum hCG levels with 77 mIU/mL as the cutoff point for oocyte retrieval.
AB - Background: Few studies have assessed the impact of serum human chorionic gonadotropin (hCG) levels before oocyte retrieval and luteinizing hormone receptor (LHR) mRNA expression. Aims: The objective was to assess the correlations between serum hCG levels at 12-h posttrigger granulosa cell LHR mRNA expression during the in vitro fertilization (IVF) cycle with oocyte maturation rate and to determine the cutoff level of serum hCG at 12-h posttrigger. Settings and Design: A cross-sectional was conducted for this study at the IVF center of Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia. Materials and Methods: Recombinant follicle-stimulating hormone was used on day 2 of the menstrual cycle with multiple doses of a gonadotropin-releasing hormone antagonist. Recombinant hCG was used to trigger ovulation. At 12-h posttrigger, hCG serum levels were measured using an enzyme-linked immunosorbent assay. Statistical Analysis: Pearson's correlation coefficient was used to evaluate the correlation between oocyte maturation rates, serum hCG levels, and LHR mRNA levels. Cutoff values were determined using a receiver operating characteristic (ROC) curve. Results: Serum hCG levels were positively correlated (r = 0.467;P < 0.01), and LHR mRNA expression was weakly correlated (r = 0.073; P = 0.701) with oocyte maturation. The cutoff of serum hCG for a high maturation rate was 77 mIU/mL, with an area under the ROC curve of 0.765 (95% confidence interval: 0.598-0.939) andP < 0.001. Conclusion: Oocyte maturation is correlated with serum hCG levels with 77 mIU/mL as the cutoff point for oocyte retrieval.
KW - Cutoff value
KW - human chorionic gonadotropin
KW - luteinizing hormone receptor
KW - oocyte maturation
KW - posttrigger
UR - http://www.scopus.com/inward/record.url?scp=85084129496&partnerID=8YFLogxK
U2 - 10.4103/jhrs.JHRS_131_19
DO - 10.4103/jhrs.JHRS_131_19
M3 - Article
AN - SCOPUS:85084129496
VL - 13
SP - 46
EP - 50
JO - Journal of Human Reproductive Sciences
JF - Journal of Human Reproductive Sciences
SN - 0974-1208
IS - 1
ER -