Immunomodulatory and antioxidant activity from Indonesian anti-degenerative herbs water extract

The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has made the number of patients gradually increase. Currently, the vaccine of SARS-CoV-2 is being researched and most of the people have to trust their immune system and being healthy as long as they could in the mean times. High antioxidant food is recommended for the people whom currently are reducing their activity from home with good exercise. Therefore, this research was focused on the Indonesian anti-degenerative herbs water extract for an immunomodulatory activity to maintain healthy productivity of lymphocyte cells and to regulate neutrophil cells production, also with antioxidant activity to maintain optimal function of the immune system. The antioxidant activity test was conducted with the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and the immunomodulator ability test was conducted with DDY-strain female mice (Mus musculus) for 28 days in five different groups. The IC50 for gallic acid and mixed herbs extract solutions was 1.89 and 146.91 μg/mL, respectively. The immunomodulatory activity was determined from the result of leukocytes differentiation which could be seen from the lymphocytes and neutrophil t-test results that were significant at p<0.1. From the results, it can be concluded that anti-degenerative extract has moderate antioxidant activity and an immunomodulator ability to regulate the productivity of lymphocyte and neutrophil cells.