TY - GEN
T1 - Immunomodulatory Activity of Propolis Powder from Tetragonula sapiens on Macrophage Function
AU - Azizah, Nurul
AU - Pratami, Diah Kartika
AU - Hermansyah, Heri
AU - Farida, Siti
AU - Sahlan, Muhamad
N1 - Funding Information:
The authors thank Lembaga Pengelola Dana Pendidikan Kementerian Keuangan RI (LPDP RI) for the RISET INOVATIF PRODUKTIF (RISPRO) grant (Grant Agreement No. PRJ-23/LPDP/2020).
Publisher Copyright:
© 2022 American Institute of Physics Inc.. All rights reserved.
PY - 2022/8/16
Y1 - 2022/8/16
N2 - Propolis is a resinous substance extracted from the plants around the honeycomb, which can be used to construct, adjust and protect the bee colony. Propolis has been known to have various benefits as traditional medicine. It contains flavonoid, caffeic acid phenethyl ester, cinnamic acid, and artepillin C, which may play a role in the immune system and promote lymphocyte proliferation. Thus, it is referred to as an immunomodulator. However, the use of liquid propolis as an active substance faces solubility problems. Propolis powder is present to overcome the limitations of the formulation of pharmaceutical preparations and can be developed as a traditional medicine with immunomodulatory activity. The purpose of this investigation was to see if propolis powder has any immunomodulatory effects using the phagocytosis macrophages method. The RAW 264.7 macrophage cell lines which had been cultured, were used in this study. Propolis samples at dosages of 100 g/ml, 50 g/ml, 25 g/ml, 12.5 g/ml, and 6.25 g/ml were used to determine the percentage of activity in RAW 264.7 macrophage cells. According to the results of this study, giving propolis powder at a dosage of 12.5 g/ml resulted in the highest macrophage capacity for phagocytosis (31±3%). In other words, propolis powder at that concentration had the greatest stimulating impact or increased phagocytosis activity the most.
AB - Propolis is a resinous substance extracted from the plants around the honeycomb, which can be used to construct, adjust and protect the bee colony. Propolis has been known to have various benefits as traditional medicine. It contains flavonoid, caffeic acid phenethyl ester, cinnamic acid, and artepillin C, which may play a role in the immune system and promote lymphocyte proliferation. Thus, it is referred to as an immunomodulator. However, the use of liquid propolis as an active substance faces solubility problems. Propolis powder is present to overcome the limitations of the formulation of pharmaceutical preparations and can be developed as a traditional medicine with immunomodulatory activity. The purpose of this investigation was to see if propolis powder has any immunomodulatory effects using the phagocytosis macrophages method. The RAW 264.7 macrophage cell lines which had been cultured, were used in this study. Propolis samples at dosages of 100 g/ml, 50 g/ml, 25 g/ml, 12.5 g/ml, and 6.25 g/ml were used to determine the percentage of activity in RAW 264.7 macrophage cells. According to the results of this study, giving propolis powder at a dosage of 12.5 g/ml resulted in the highest macrophage capacity for phagocytosis (31±3%). In other words, propolis powder at that concentration had the greatest stimulating impact or increased phagocytosis activity the most.
KW - immunomodulator
KW - phagocytosis
KW - propolis powder
KW - Tetragonula sapiens
UR - http://www.scopus.com/inward/record.url?scp=85138307199&partnerID=8YFLogxK
U2 - 10.1063/5.0098749
DO - 10.1063/5.0098749
M3 - Conference contribution
AN - SCOPUS:85138307199
T3 - AIP Conference Proceedings
BT - 6th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices
A2 - Rahman, Siti Fauziyah
A2 - Zakiyuddin, Ahmad
A2 - Whulanza, Yudan
A2 - Intan, Nurul
PB - American Institute of Physics Inc.
T2 - 6th International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2021
Y2 - 7 July 2021 through 8 July 2021
ER -