TY - GEN
T1 - Immunomodulatory Activity Based on Total Protein Serum from Indonesian Anti-neurodegenerative Mixed-herb Water Extract
AU - Tristantini, Dewi
AU - Karlinda, Nabila Larasati
AU - Santoso, Leon Lukhas
N1 - Funding Information:
The authors thank all research staff, lecturers, and academic staff in the Department of Chemical Engineering, Universitas Indonesia, for providing the necessary facilities to carry out this study. The authors also thank Keisha Nabila Zalfa Zahirah and Millennie Fitria from the Department of Chemical Engineering, Universitas Indonesia for their help in writing the manuscript. This research is partially funded by the Applied Research BRIN Program (NKB-252/UN2.RST/HKP.05.00/2021), managed by the Directorate of Research and Development Universitas Indonesia.
Publisher Copyright:
© 2022 American Institute of Physics Inc.. All rights reserved.
PY - 2022/8/16
Y1 - 2022/8/16
N2 - Modern lifestyle has made many live in an unhealthy diet and high working stress. Those conditions make a high possibility for anyone to compromise their immune system. In the past, people have used herbal medicine to maintain their health. One of the medicines from Central Java, Indonesia has been used to reduce neural pain and enhanced the immune system for generations. The formula consists of cloves (Syzygium aromaticum), ginger (Zingiber officinale), and nutmeg (Myristica fragrans) with water as the solvent. In this research, the formula's extract was tested for immunomodulatory activity based on total protein serum in mice (Mus musculus). The extraction process used the reflux method with 10 g of dried sample in 250 ml of water at a temperature of 80°C for 90 minutes. The immunomodulatory test was conducted with five mice groups: the normal group, the positive group with immune booster medicine, and three dosage groups derived from 62.5, 125, and 250 mL of mixed-herb extract for 28 days. The result has shown that the formula's extract has high effectiveness in enhancing the total protein amount, which is 28.11% for the lowest dosage and 37.64% for the most significant dosage. Compared to the positive group, the formula's extract tended to increase globulin rather than albumin. Those significant phenomena have also been proven from ANOVA single-factor analysis with 0.05 of confidence level, resulting in a p-value of 0.001. Therefore, the Indonesian Anti-neurodegenerative mixed-herb water extract has been proven to have a high potential as an immunomodulator.
AB - Modern lifestyle has made many live in an unhealthy diet and high working stress. Those conditions make a high possibility for anyone to compromise their immune system. In the past, people have used herbal medicine to maintain their health. One of the medicines from Central Java, Indonesia has been used to reduce neural pain and enhanced the immune system for generations. The formula consists of cloves (Syzygium aromaticum), ginger (Zingiber officinale), and nutmeg (Myristica fragrans) with water as the solvent. In this research, the formula's extract was tested for immunomodulatory activity based on total protein serum in mice (Mus musculus). The extraction process used the reflux method with 10 g of dried sample in 250 ml of water at a temperature of 80°C for 90 minutes. The immunomodulatory test was conducted with five mice groups: the normal group, the positive group with immune booster medicine, and three dosage groups derived from 62.5, 125, and 250 mL of mixed-herb extract for 28 days. The result has shown that the formula's extract has high effectiveness in enhancing the total protein amount, which is 28.11% for the lowest dosage and 37.64% for the most significant dosage. Compared to the positive group, the formula's extract tended to increase globulin rather than albumin. Those significant phenomena have also been proven from ANOVA single-factor analysis with 0.05 of confidence level, resulting in a p-value of 0.001. Therefore, the Indonesian Anti-neurodegenerative mixed-herb water extract has been proven to have a high potential as an immunomodulator.
KW - Albumin
KW - Anti-neurodegenerative
KW - Globulin
KW - Herbal medicine
KW - Immunomodulator
UR - http://www.scopus.com/inward/record.url?scp=85138222835&partnerID=8YFLogxK
U2 - 10.1063/5.0097886
DO - 10.1063/5.0097886
M3 - Conference contribution
AN - SCOPUS:85138222835
T3 - AIP Conference Proceedings
BT - 6th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices
A2 - Rahman, Siti Fauziyah
A2 - Zakiyuddin, Ahmad
A2 - Whulanza, Yudan
A2 - Intan, Nurul
PB - American Institute of Physics Inc.
T2 - 6th International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2021
Y2 - 7 July 2021 through 8 July 2021
ER -