Immobilization of Candida rugosa lipase by adsorption-crosslinking onto corn husk

A. Nuraliyah, A. Wijanarko, H. Hermansyah

Research output: Contribution to journalConference articlepeer-review

6 Citations (Scopus)

Abstract

Corn husk is one of the agricutural waste that has not been used optimally. corn husk waste allows to be used as immobilized support for biocatalyst because it is easy to obtain, available abundant, renewable and easy to decompose. This research was conducted in two phases, namely the adsorption of enzyme immobilization on the support, followed by cross- linking between the enzyme and support through the addition of glutaraldehyde. The optimum conditions for cross-linked adsorption immobilization using support of corn husk were achieved at concentrations of 0,75 mg / ml at 4 hour reaction time. The biggest unit activity value is obtained at 2,37 U / g support through 0.5% glutaraldehyde addition.

Original languageEnglish
Article number012042
JournalIOP Conference Series: Materials Science and Engineering
Volume345
Issue number1
DOIs
Publication statusPublished - 12 Apr 2018
Event2nd International Conference on Oleo and Petrochemical Engineering, ICOOPChE 2017 - Pekanbaru-Riau, Indonesia
Duration: 29 Nov 201730 Nov 2017

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