TY - JOUR
T1 - Immobilization of Candida rugosa lipase by adsorption-crosslinking onto corn husk
AU - Nuraliyah, A.
AU - Wijanarko, A.
AU - Hermansyah, H.
N1 - Publisher Copyright:
© Published under licence by IOP Publishing Ltd.
PY - 2018/4/12
Y1 - 2018/4/12
N2 - Corn husk is one of the agricutural waste that has not been used optimally. corn husk waste allows to be used as immobilized support for biocatalyst because it is easy to obtain, available abundant, renewable and easy to decompose. This research was conducted in two phases, namely the adsorption of enzyme immobilization on the support, followed by cross- linking between the enzyme and support through the addition of glutaraldehyde. The optimum conditions for cross-linked adsorption immobilization using support of corn husk were achieved at concentrations of 0,75 mg / ml at 4 hour reaction time. The biggest unit activity value is obtained at 2,37 U / g support through 0.5% glutaraldehyde addition.
AB - Corn husk is one of the agricutural waste that has not been used optimally. corn husk waste allows to be used as immobilized support for biocatalyst because it is easy to obtain, available abundant, renewable and easy to decompose. This research was conducted in two phases, namely the adsorption of enzyme immobilization on the support, followed by cross- linking between the enzyme and support through the addition of glutaraldehyde. The optimum conditions for cross-linked adsorption immobilization using support of corn husk were achieved at concentrations of 0,75 mg / ml at 4 hour reaction time. The biggest unit activity value is obtained at 2,37 U / g support through 0.5% glutaraldehyde addition.
UR - http://www.scopus.com/inward/record.url?scp=85046280012&partnerID=8YFLogxK
U2 - 10.1088/1757-899X/345/1/012042
DO - 10.1088/1757-899X/345/1/012042
M3 - Conference article
AN - SCOPUS:85046280012
SN - 1757-8981
VL - 345
JO - IOP Conference Series: Materials Science and Engineering
JF - IOP Conference Series: Materials Science and Engineering
IS - 1
M1 - 012042
T2 - 2nd International Conference on Oleo and Petrochemical Engineering, ICOOPChE 2017
Y2 - 29 November 2017 through 30 November 2017
ER -