TY - JOUR
T1 - Immense addition of royal jelly apis mellifera (ceiba pentandra) insufficient to increase fibroblast preputium proliferation
AU - Pramono, A.
AU - Bustamam, N.
AU - Amalia, M.
AU - Sahlan, M.
PY - 2019/5/1
Y1 - 2019/5/1
N2 - Culture of preputium skin fibroblasts is a good cell source in the manufacture of induced pluripotent stem cells (iPS). Induced pluripotent stem cells (induced pluripotent stem cell / iPSC) is the latest stem cell technology to get the source of stem cells that are equivalent to their pluripotent properties with embryonic cells, without having to consider ethical problems that arise. Culture systems of preputium skin fibroblast, can use a variety of media. Currently, Dulbeccos modified Eagle medium (DMEM) plus Fetal Bovine Serum (FBS), but if the culture is carried out continuously in FBS, it can increase the immune systems resistance. The addition of royal jelly 5 mg / ml of culture medium was not sufficient to increase the fibroblast preputium viability (p=0,05) so that further research to optimize FBS substitute culture medium is needed. This study was conducted to analyze the comparison of the effectiveness of serum free DMEM media with the addition of Apis Mellifera royal jelly bee from Ceiba pentandra flower for preputium skin fibroblasts cell culture. Royal jelly addition in 1%, 2%, and 5% showed less effect in the proliferation of fibroblast cell, compare with serum addition. The optimum medium for Fetal Bovine Serum (FBS) substitution in skin fibroblast cell cultures must continue to be developed, especially for the transduction protein medium. The results of this study are expected to be used to improve transduction protein medium in iPSC engineering.
AB - Culture of preputium skin fibroblasts is a good cell source in the manufacture of induced pluripotent stem cells (iPS). Induced pluripotent stem cells (induced pluripotent stem cell / iPSC) is the latest stem cell technology to get the source of stem cells that are equivalent to their pluripotent properties with embryonic cells, without having to consider ethical problems that arise. Culture systems of preputium skin fibroblast, can use a variety of media. Currently, Dulbeccos modified Eagle medium (DMEM) plus Fetal Bovine Serum (FBS), but if the culture is carried out continuously in FBS, it can increase the immune systems resistance. The addition of royal jelly 5 mg / ml of culture medium was not sufficient to increase the fibroblast preputium viability (p=0,05) so that further research to optimize FBS substitute culture medium is needed. This study was conducted to analyze the comparison of the effectiveness of serum free DMEM media with the addition of Apis Mellifera royal jelly bee from Ceiba pentandra flower for preputium skin fibroblasts cell culture. Royal jelly addition in 1%, 2%, and 5% showed less effect in the proliferation of fibroblast cell, compare with serum addition. The optimum medium for Fetal Bovine Serum (FBS) substitution in skin fibroblast cell cultures must continue to be developed, especially for the transduction protein medium. The results of this study are expected to be used to improve transduction protein medium in iPSC engineering.
UR - http://www.scopus.com/inward/record.url?scp=85065984268&partnerID=8YFLogxK
U2 - 10.1088/1757-899X/508/1/012145
DO - 10.1088/1757-899X/508/1/012145
M3 - Conference article
AN - SCOPUS:85065984268
VL - 508
JO - IOP Conference Series: Materials Science and Engineering
JF - IOP Conference Series: Materials Science and Engineering
SN - 1757-8981
IS - 1
M1 - 012145
T2 - 1st Tarumanagara International Conference on the Applications of Technology and Engineering, TICATE 2018
Y2 - 22 November 2018 through 23 November 2018
ER -